https://scholars.lib.ntu.edu.tw/handle/123456789/462219
標題: | MicroRNA-17/20a functions to inhibit cell migration and can be used a prognostic marker in oral squamous cell carcinoma | 作者: | Chang C.-C. Yang Y.-J. Li Y.-J. Chen S.-T. BEEN-REN LIN Wu T.-S. SZE-KWAN LIN YEN-PING KUO CHING-TING TAN |
公開日期: | 2013 | 出版社: | Elsevier Ltd | 卷: | 49 | 期: | 9 | 起(迄)頁: | 923-931 | 來源出版物: | Oral Oncology | 摘要: | Objectives Oral squamous cell carcinoma (OSCC) accounts for >90% oral cancer which is a leading cause of cancer death worldwide. Early diagnosis may well offer an opportunity to increase survival to this neoplasm. Micro(mi)RNA-interfered cancer progression is crucial, yet its migration machinery of OSCC is still unknown. To access whether the possible miRNA prognostic markers and underlying mechanisms, we developed a highly migratory TW2.6 MS-10 cells from TW2.6 cells to investigate the issue. Materials and methods miRNA profiling was performed on TW2.6 and TW2.6 MS-10. Target miRNA was correlated to pathological status in OSCC patients by real-time RT-PCR. A downstream effector was identified using a bioinformatics analysis, and a 3′-untranslated region (UTR) reporter assay was used. Results An miRNA cluster, miR-17-92, including miR-17, miR-19b, miR-20a, and miR-92a, was found to be significantly down-regulated in TW2.6 MS-10 compared to TW2.6 cells. Overexpression of this cluster decreased the migratory ability of OSCC cell lines. We further demonstrated that miR-17 and miR-20a are the main miRNAs of miR-17-92 cluster which modulate OSCC migration. Clinically, miR-17/20a showed negative correlation with TNM stage and lymphatic metastasis. Through a bioinformatics screening analysis and 3′UTR reporter assay, we confirmed the integrin (ITG) β8 as a direct target of miR-17/20a, and knockdown of ITGβ8 reduced cell migratory capability of OSCC. Conclusions miR-17/20a acts as a prognostic predictor of OSCC patients' outcome and a tumor migration suppressor miRNA. ? 2013 Elsevier Ltd. All rights reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84883135588&doi=10.1016%2fj.oraloncology.2013.03.430&partnerID=40&md5=69fce08c839654afd267ac9c3efcc3a0 https://scholars.lib.ntu.edu.tw/handle/123456789/462219 |
ISSN: | 1368-8375 | DOI: | 10.1016/j.oraloncology.2013.03.430 | SDG/關鍵字: | beta integrin; beta8 integrin; microRNA; microRNA 17; microRNA 19b; microRNA 20a; microrna 92a; unclassified drug; 3' untranslated region; article; cancer cell; cancer prognosis; cancer staging; cell migration; controlled study; down regulation; gene expression; human; human cell; lymph node metastasis; major clinical study; mouth squamous cell carcinoma; priority journal; reverse transcription polymerase chain reaction; 3′ untranslated region; 3′UTR; comparative threshold; CT; ingenuity pathway analysis; integrin β8; IPA; ITGβ8; LAMA3; laminin α3; microRNA; Migration; miRNA; miRNA-17; miRNA-20a; oral squamous cell carcinoma; Oral squamous cell carcinoma; OSCC; receiver-operating characteristics; ROC; Carcinoma, Squamous Cell; Cell Line, Tumor; Cell Movement; Cluster Analysis; Humans; MicroRNAs; Mouth Neoplasms; Prognosis |
顯示於: | 醫學系 |
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