https://scholars.lib.ntu.edu.tw/handle/123456789/462219
DC Field | Value | Language |
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dc.contributor.author | Chang C.-C. | en_US |
dc.contributor.author | Yang Y.-J. | en_US |
dc.contributor.author | Li Y.-J. | en_US |
dc.contributor.author | Chen S.-T. | en_US |
dc.contributor.author | BEEN-REN LIN | en_US |
dc.contributor.author | Wu T.-S. | en_US |
dc.contributor.author | SZE-KWAN LIN | en_US |
dc.contributor.author | YEN-PING KUO | en_US |
dc.contributor.author | CHING-TING TAN | en_US |
dc.creator | Chang C.-C.;Yang Y.-J.;Li Y.-J.;Chen S.-T.;Been-Ren Lin;Wu T.-S.;Lin S.-K.;Kuo M.Y.-P.;Tan C.-T. | - |
dc.date.accessioned | 2020-02-24T02:29:19Z | - |
dc.date.available | 2020-02-24T02:29:19Z | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 1368-8375 | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84883135588&doi=10.1016%2fj.oraloncology.2013.03.430&partnerID=40&md5=69fce08c839654afd267ac9c3efcc3a0 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/462219 | - |
dc.description.abstract | Objectives Oral squamous cell carcinoma (OSCC) accounts for >90% oral cancer which is a leading cause of cancer death worldwide. Early diagnosis may well offer an opportunity to increase survival to this neoplasm. Micro(mi)RNA-interfered cancer progression is crucial, yet its migration machinery of OSCC is still unknown. To access whether the possible miRNA prognostic markers and underlying mechanisms, we developed a highly migratory TW2.6 MS-10 cells from TW2.6 cells to investigate the issue. Materials and methods miRNA profiling was performed on TW2.6 and TW2.6 MS-10. Target miRNA was correlated to pathological status in OSCC patients by real-time RT-PCR. A downstream effector was identified using a bioinformatics analysis, and a 3′-untranslated region (UTR) reporter assay was used. Results An miRNA cluster, miR-17-92, including miR-17, miR-19b, miR-20a, and miR-92a, was found to be significantly down-regulated in TW2.6 MS-10 compared to TW2.6 cells. Overexpression of this cluster decreased the migratory ability of OSCC cell lines. We further demonstrated that miR-17 and miR-20a are the main miRNAs of miR-17-92 cluster which modulate OSCC migration. Clinically, miR-17/20a showed negative correlation with TNM stage and lymphatic metastasis. Through a bioinformatics screening analysis and 3′UTR reporter assay, we confirmed the integrin (ITG) β8 as a direct target of miR-17/20a, and knockdown of ITGβ8 reduced cell migratory capability of OSCC. Conclusions miR-17/20a acts as a prognostic predictor of OSCC patients' outcome and a tumor migration suppressor miRNA. ? 2013 Elsevier Ltd. All rights reserved. | - |
dc.publisher | Elsevier Ltd | - |
dc.relation.ispartof | Oral Oncology | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | beta integrin; beta8 integrin; microRNA; microRNA 17; microRNA 19b; microRNA 20a; microrna 92a; unclassified drug; 3' untranslated region; article; cancer cell; cancer prognosis; cancer staging; cell migration; controlled study; down regulation; gene expression; human; human cell; lymph node metastasis; major clinical study; mouth squamous cell carcinoma; priority journal; reverse transcription polymerase chain reaction; 3′ untranslated region; 3′UTR; comparative threshold; CT; ingenuity pathway analysis; integrin β8; IPA; ITGβ8; LAMA3; laminin α3; microRNA; Migration; miRNA; miRNA-17; miRNA-20a; oral squamous cell carcinoma; Oral squamous cell carcinoma; OSCC; receiver-operating characteristics; ROC; Carcinoma, Squamous Cell; Cell Line, Tumor; Cell Movement; Cluster Analysis; Humans; MicroRNAs; Mouth Neoplasms; Prognosis | - |
dc.title | MicroRNA-17/20a functions to inhibit cell migration and can be used a prognostic marker in oral squamous cell carcinoma | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1016/j.oraloncology.2013.03.430 | - |
dc.identifier.pmid | 23602254 | - |
dc.identifier.scopus | 2-s2.0-84883135588 | - |
dc.relation.pages | 923-931 | - |
dc.relation.journalvolume | 49 | - |
dc.relation.journalissue | 9 | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
crisitem.author.dept | Surgery-NTUH | - |
crisitem.author.dept | Surgery | - |
crisitem.author.dept | Dentistry | - |
crisitem.author.dept | School of Dentistry | - |
crisitem.author.dept | Dentistry-NTUH | - |
crisitem.author.dept | Dentistry | - |
crisitem.author.dept | School of Dentistry | - |
crisitem.author.dept | Dentistry-NTUH | - |
crisitem.author.dept | Otolaryngology | - |
crisitem.author.dept | Otolaryngology-NTUH | - |
crisitem.author.orcid | 0000-0001-8354-1758 | - |
crisitem.author.orcid | 0000-0002-7969-0280 | - |
crisitem.author.orcid | 0000-0001-8321-2575 | - |
crisitem.author.orcid | 0000-0001-8317-2235 | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
Appears in Collections: | 醫學系 |
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