https://scholars.lib.ntu.edu.tw/handle/123456789/462446
標題: | Identification of GRP75 as an independent favorable prognostic marker of neuroblastoma by a proteomics analysis | 作者: | WEN-MING HSU HSIN-YU LEE HSUEH-FEN JUAN Shih Y.-Y. Wang B.-J. CHIEN-YUAN PAN YUNG-MING JENG HSIU-HAO CHANG MENG-YAO LU Lin K.-H. HONG-SHIEE LAI Chen W.-J. Tsay Y.-G. Liao Y.-F. FON-JOU HSIEH |
公開日期: | 2008 | 卷: | 14 | 期: | 19 | 起(迄)頁: | 6237-6245 | 來源出版物: | Clinical Cancer Research | 摘要: | Purpose: Neuroblastoma (NB) is a heterogeneous neoplasm. Detailed biological discrimination is critical for the effective treatment of this disease. Because the tumor behavior of NB is closely associated with the histologic state of differentiation, we thus aimed to identify novel differentiation-associated markers of NB with prognostic implication. Experimental Design: A human NB cell line SH-SY5Y was used as a model system to explore potential biomarkers for the differentiation of NB by proteomic analyses. Seventy-two NB tumor tissues were subsequently investigated by immunohistochemistry to validate the correlations between the expression of a novel prognostic marker, various clinicopathologic and biological factors, and patient survival. Results: Using two-dimensional differential gel electrophoresis, we found a total of 24 spots of proteins in SH-SY5Y cells whose expression was enhanced following differentiation. Glucose-regulated protein 75 (GRP75) was unambiguously identified as one of the five proteins that were dramatically up-regulated following differentiation. Immunohistochemical analyses of 72 NB tumor tissues further revealed that positive GRP75 immunostaining is strongly correlated with differentiated histologies (P < 0.001), mass-screened tumors (P = 0.016), and early clinical stages (P < 0.001) but inversely correlated with MYCN amplification (P = 0.010). Univariate and multivariate survival analyses showed that GRP75 expressionis an independent favorable prognostic factor. Conclusions: The present findings clearly showed that our proteomics-based novel experimental paradigm could be a powerful tool to uncover novel biomarkers associated with the differentiation of NB. Our data also substantiate an essential role of GRP75 in the differentiation of NB. ?2008 American Association for Cancer Research. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-58149157945&doi=10.1158%2f1078-0432.CCR-07-4181&partnerID=40&md5=ad07385e9e842634d89560d8f8d36e59 https://scholars.lib.ntu.edu.tw/handle/123456789/462446 |
ISSN: | 1078-0432 | DOI: | 10.1158/1078-0432.CCR-07-4181 | SDG/關鍵字: | biological factor; glucose regulated protein; glucose regulated protein 75; tumor marker; unclassified drug; glucose regulated proteins; glucose-regulated proteins; heat shock protein 70; membrane protein; proteome; tumor marker; article; cancer staging; cancer survival; cell differentiation; cellular distribution; clinical feature; controlled study; histopathology; human; human cell; human tissue; immunohistochemistry; neuroblastoma; priority journal; prognosis; protein expression; protein synthesis; proteomics; two dimensional gel electrophoresis; child; female; gene expression regulation; infant; male; metabolism; methodology; neuroblastoma; newborn; preschool child; proteomics; tumor cell line; Cell Differentiation; Cell Line, Tumor; Child; Child, Preschool; Female; Gene Expression Regulation, Neoplastic; HSP70 Heat-Shock Proteins; Humans; Infant; Infant, Newborn; Male; Membrane Proteins; Neuroblastoma; Prognosis; Proteome; Proteomics; Tumor Markers, Biological |
顯示於: | 醫學系 |
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