https://scholars.lib.ntu.edu.tw/handle/123456789/465441
Title: | Electrotaxis of lung cancer cells in a multiple-electric-field chip | Authors: | Huang C.-W Cheng J.-Y Yen M.-H Tai-Horng Young |
Keywords: | Cancer metastasis; Electrotaxis; Lung cancer cell; Metastasis-electrotaxis; Microfluidic | Issue Date: | 2009 | Journal Volume: | 24 | Journal Issue: | 12 | Start page/Pages: | 3510-3516 | Source: | Biosensors and Bioelectronics | Abstract: | We report a microfluidic cell culture chip that was used for long-term electrotaxis study on a microscope. The cellular response under three different electric field strengths was studied in a single channel microfluidic chip. Electric field (EF) inside the microchamber was numerically simulated and compared to the measured value. Lung cancer cell lines with high and weak metastasis potential, CL1-5 and CL1-0, respectively, were used to demonstrate the function of the multi-field chip (MFC). The two cell lines exhibited greatly different response under the applied EF of E = 74-375 mV/mm. CL1-5 cells migrated toward the anode while CL1-0 cells did not show obvious response. Under the applied EF, cell orientation was observed accompanying the cell migration. Judging from the different temporal responses of the orientation and the migration, it is proposed that the two EF-induced responses may involve different signaling pathways. ? 2009 Elsevier B.V. All rights reserved. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/465441 | ISSN: | 0956-5663 | DOI: | 10.1016/j.bios.2009.05.001 | SDG/Keyword: | Cancer metastasis; Cell lines; Cell migration; Cell orientation; Cellular response; Electric field strength; Electrotaxis; Lung cancer cell; Lung cancer cells; Metastasis-electrotaxis; Micro-chamber; Microfluidic cell culture; Microfluidic chip; Multi-field; Signaling pathways; Single channels; Temporal response; Biochips; Biological organs; Cells; Electric field measurement; Electric fields; Pathology; Cell culture; animal cell; article; cancer cell; cancer cell culture; cell migration; electric field; electric potential; human; human cell; lung cancer; metastasis potential; microfluidic analysis; microfluidics; mouse; nonhuman; rat; signal transduction; simulation; Cell Culture Techniques; Cell Line, Tumor; Cell Movement; Cell Separation; Electrochemistry; Humans; Lung Neoplasms; Microfluidic Analytical Techniques; Micromanipulation; Radiation Dosage |
Appears in Collections: | 醫學工程學研究所 |
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