https://scholars.lib.ntu.edu.tw/handle/123456789/467585
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Han, H.-W. | en_US |
dc.contributor.author | Hsu, S.-H. | en_US |
dc.contributor.author | SHAN-HUI HSU | en_US |
dc.creator | SHAN-HUI HSU;Hsu, S.-H.;Han, H.-W. | - |
dc.date.accessioned | 2020-03-02T07:41:23Z | - |
dc.date.available | 2020-03-02T07:41:23Z | - |
dc.date.issued | 2016 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/467585 | - |
dc.description.abstract | The controversial roles of mesenchymal stem cells (MSCs) in lung cancer development are not yet resolved because of the lack of an extracellular environment that mimics the tumor microenvironment. Three-dimensional (3D) culture system is an emerging research tool for biomedical applications such as drug screening. In this study, MSCs and human non-small cell lung carcinoma cells (A549) were co-cultured on a thin biomaterial-based substratum (hyaluronan-grafted chitosan, CS-HA; ?2?μm), and they were self-organized into the 3D tumor co-spheroids with core-shell structure. The gene expression levels of tumorigenicity markers in cancer cells associated with cancer stemness, epithelial-mesenchymal transition (EMT) property, and cell mobility were up-regulated for more than twofold in the MSC-tumor co-spheroids, through the promoted expression of certain tumor enhancers and the direct cell-cell interaction. To verify the different extents of tumorigenicity, A549 cells or those co-cultured with MSCs were transplanted into zebrafish embryos for evaluation in vivo. The tumorigenicity obtained from the zebrafish xenotransplantation model was consistent with that observed in vitro. These evidences suggest that the CS-HA substrate-based 3D co-culture platform for cancer cells and MSCs may be a convenient tool for studying the cell-cell interaction in a tumor-like microenvironment and potentially for cancer drug testing. Statement of Significance Mesenchymal stem cells (MSCs) have been found in several types of tumor tissues. However, the controversial roles of MSCs in cancer development are still unsolved. Chitosan and hyaluronan are commonly used materials in the biomedical field. In the current study, we co-cultured lung cancer cells and MSCs on the planar hyaluronan-grafted chitosan (CS-HA) hybrid substrates, and discovered that lung cancer cells and MSCs were rapidly self-assembled into 3D tumor spheroids with core-shell structure on the substrates after only two days in culture. Therefore, CS-HA based 3D co-culture platform can be applied to exploration of the relationship between cancer cells and MSCs and other cancer-related medical applications such as drug screening. ? 2016 Acta Materialia Inc. | - |
dc.relation.ispartof | Acta Biomaterialia | - |
dc.subject | 3D culture; Chitosan-hyaluronan; Lung cancer; Mesenchymal stem cells; Zebrafish transplantation | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | Biological organs; Cell culture; Crosstalk; Diagnosis; Diseases; Enzyme activity; Flowcharting; Gene expression; Hyaluronic acid; Medical applications; Shells (structures); Stem cells; Substrates; Tumors; 3-d cultures; Chitosan-hyaluronan; Co-cultures; Hyaluronan; Lung Cancer; Lung cancer cells; Mesenchymal stem cell; Tumorigenicity; Zebrafish; Zebrafish transplantation; Chitosan; CD33 antigen; chitosan; fibronectin; gelatinase A; gelatinase B; Hermes antigen; hyaluronic acid; interleukin 10; nerve cell adhesion molecule; stromal cell derived factor 1; transcription factor NANOG; transcription factor Snail; transcription factor Sox2; transcription factor Twist; transforming growth factor beta1; tumor marker; chitosan; conditioned medium; hyaluronic acid; tumor marker; animal experiment; animal model; Article; cancer cell; cell interaction; cell motility; epithelial mesenchymal transition; gene expression; human; human cell; lung carcinogenesis; mesenchymal stem cell; non small cell lung cancer; nonhuman; priority journal; tumor microenvironment; tumor spheroid; tumor xenograft; upregulation; zebra fish; A-549 cell line; animal; cancer stem cell; carcinogenesis; cell count; cell motion; cell size; coculture; conditioned medium; cytology; drug effects; drug screening; gene expression regulation; genetics; lung tumor; mesenchymal stroma cell; metabolism; multicellular spheroid; pathology; pharmacology; procedures; reproducibility; A549 Cells; Animals; Biomarkers, Tumor; Carcinogenesis; Cell Count; Cell Movement; Cell Size; Chitosan; Coculture Techniques; Culture Media, Conditioned; Epithelial-Mesenchymal Transition; Gene Expression Regulation, Neoplastic; Hyaluronic Acid; Lung Neoplasms; Mesenchymal Stromal Cells; Neoplastic Stem Cells; Reproducibility of Results; Spheroids, Cellular; Xenograft Model Antitumor Assays; Zebrafish | - |
dc.title | Chitosan-hyaluronan based 3D co-culture platform for studying the crosstalk of lung cancer cells and mesenchymal stem cells | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1016/j.actbio.2016.06.014 | - |
dc.identifier.scopus | 2-s2.0-84983313741 | - |
dc.identifier.url | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84983313741&doi=10.1016%2fj.actbio.2016.06.014&partnerID=40&md5=de4dfcc13ca05aa8d05562bafb13883f | - |
dc.relation.pages | 157-167 | - |
dc.relation.journalvolume | 42 | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
crisitem.author.dept | Polymer Science and Engineering | - |
crisitem.author.orcid | 0000-0003-3399-055X | - |
crisitem.author.parentorg | College of Engineering | - |
顯示於: | 高分子科學與工程學研究所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。