https://scholars.lib.ntu.edu.tw/handle/123456789/468749
Title: | Autologous Mesenchymal stem cells prevent transplant arteriosclerosis by enhancing local expression of interleukin-10, interferon-γ, and indoleamine 2,3-dioxygenase | Authors: | Jui, Hsiang-Yiang Lin, Cheng-Hsin WAN-TSENG HSU Liu, Yi-Ru RON-BIN HSU BOR-LUEN CHIANG WEN-YIH TSENG Chen, Ming-Fong Wu, Kenneth K. CHII-MING LEE |
Keywords: | Immunomodulation; Mesenchymal stem cells (MSCs); Transplant arteriosclerosis | Issue Date: | 2012 | Journal Volume: | 21 | Journal Issue: | 5 | Start page/Pages: | 971-984 | Source: | Cell Transplantation | Abstract: | Transplant arteriosclerosis (TA) remains the major limitation of long-term graft survival in heart transplantation despite the advances in immunosuppressants. Mesenchymal stem cells (MSCs) have been demonstrated to suppress allogeneic immune responses by numerous in vitro studies. However, the immunomodulatory effects of MSCs in vivo are controversial and the underlying molecular mechanisms are not conclusive. In this study, we investigated the therapeutic potential of autologous bone marrow-derived MSCs on TA in a porcine model of femoral artery transplantation. MSCs or saline were injected into the soft tissue surrounding the arterial grafts immediately postanastomosis. Four weeks after transplantation, neointimal formation increased significantly in untreated allografts compared with the MSC-treated grafts as assessed by intravascular ultrasound (maximum luminal area stenosis: 40 ± 12% vs. 18 ± 6%, p < 0.001). Grafts harvested at 4 weeks showed dense perivascular lymphocyte infiltration accompanied by significant intimal hyperplasia in the untreated but not in the MSC-treated allografts. Serial angiographic examination showed that all of the untreated allografts became occluded at the 8th week whereas the majority of the MSC-treated grafts remained patent at the 12th week posttransplantation (n = 12 each group, p < 0.001). Quantitative PCR analysis revealed that Foxp3 expression was comparable between the untreated and the MSC-treated groups. However, expression of interleukin-10 (IL-10), interferon-γ (IFN-γ), and indoleamine 2,3-dioxygenase (IDO) was increased significantly in the MSC-treated allografts compared with that in the allograft controls (p = 0.021 for IL-10, p = 0.003 for IFN-γ, and p = 0.008 for IDO). In conclusion, local delivery of autologous MSCs alleviates TA by inducing allograft tolerance via enhanced expression of IL-10, IFN-γ, and IDO but not Foxp3-positive cells in the vessel wall. These results suggest that MSCs induce immune tolerance by activating the type 1 regulatory T-like cells. © 2012 Cognizant Comm. Corp. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84862536061&doi=10.3727%2f096368911X627525&partnerID=40&md5=1899cb7397931bbef133939c1a6cd45b https://scholars.lib.ntu.edu.tw/handle/123456789/468749 |
ISSN: | 0963-6897 | DOI: | 10.3727/096368911X627525 |
Appears in Collections: | 醫療器材與醫學影像研究所 |
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