https://scholars.lib.ntu.edu.tw/handle/123456789/468836
Title: | Cilostazol Attenuates Retinal Oxidative Stress and Inflammation in a Streptozotocin-Induced Diabetic Animal Model | Authors: | PO-TING YEH Huang Y.-H. SHU-WEN CHANG Wang L.-C. CHUNG-MAY YANG WEI-SHIUNG YANG CHUNG-WU LIN CHANG-HAO YANG |
Issue Date: | 2019 | Publisher: | Taylor and Francis Ltd | Journal Volume: | 44 | Journal Issue: | 3 | Start page/Pages: | 294-302 | Source: | Current Eye Research | Abstract: | Purpose: To investigate the anti-oxidative and anti-inflammatory effects of cilostazol in the ocular tissues of streptozotocin (STZ)-induced diabetic rats. Materials and methods: Diabetes was induced in 6-week-old Wistar rats via peritoneal injections of STZ. The treatment group received cilostazol 18 mg/kg/day for 8?weeks (n =?10), and the diabetic group received phosphate buffer solution (n =?20). The expression of oxidative stress and inflammatory mediators in the ocular tissues was then assessed by reverse-transcription polymerase chain reactions, immunohistochemical (IHC) staining, Western blot analysis, and enzyme-linked immunosorbent assay (ELISA). Results: Reverse-transcription polymerase chain reactions, IHC staining, Western blot analysis, and ELISA showed that cilostazol inhibited mRNA and protein expressions of intercellular adhesion molecule-1, monocyte chemoattractant protein-1 and fractalkine in the retina and aqueous humor (AqH). Consistent with these findings, cilostazol attenuated the activation of nuclear factor-κB (NF-κB) in the diabetic rats. The levels of oxidatively modified DNA (8-OHdG), nitrotyrosine and oxidative lipids (acrolein) were also diminished in the cilostazol-treated group. Chemiluminescence analysis showed that reactive oxygen species (ROS) levels in the AqH was significantly higher in the diabetic rats than in the non-diabetic rats. Treatment with cilostazol significantly reduced the ROS levels in the AqH compared to the diabetic rats. Conclusions: Our results indicated that cilostazol reduced inflammatory reactions and oxidative stress in diabetic eyes. The anti-inflammatory effects of cilostazol may be indirectly via reducing oxidative stress, inhibiting NF-κB activity, and subsequently decreasing inflammatory mediators. Cilostazol may be beneficial to prevent the progression of diabetic retinopathy. ? 2018, ? 2018 Taylor & Francis Group, LLC. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85057321441&doi=10.1080%2f02713683.2018.1542734&partnerID=40&md5=2bd2776be954c98d46e320c3d060b8c8 https://scholars.lib.ntu.edu.tw/handle/123456789/468836 |
ISSN: | 0271-3683 | DOI: | 10.1080/02713683.2018.1542734 | SDG/Keyword: | 3 nitrotyrosine; acrolein; cilostazol; DNA; fractalkine; glucose; immunoglobulin enhancer binding protein; intercellular adhesion molecule 1; messenger RNA; monocyte chemotactic protein 1; placebo; reactive oxygen metabolite; streptozocin; Ccl2 protein, rat; cilostazol; Cx3cl1 protein, rat; fractalkine; ICAM1 protein, rat; immunoglobulin enhancer binding protein; intercellular adhesion molecule 1; monocyte chemotactic protein 1; phosphodiesterase III inhibitor; reactive oxygen metabolite; animal experiment; animal model; antiinflammatory activity; antioxidant activity; aqueous humor; Article; body weight; chemoluminescence; controlled study; diabetic retinopathy; enzyme linked immunosorbent assay; eye tissue; female; gel mobility shift assay; glucose blood level; immunohistochemistry; mRNA expression level; nonhuman; oxidative stress; priority journal; protein expression level; rat; retina tissue; reverse transcription polymerase chain reaction; streptozotocin-induced diabetes mellitus; Western blotting; animal; diabetic retinopathy; drug effect; experimental diabetes mellitus; gene expression regulation; genetics; inflammation; metabolism; oxidative stress; physiology; retina; Wistar rat; Animals; Aqueous Humor; Blood Glucose; Blotting, Western; Chemokine CCL2; Chemokine CX3CL1; Cilostazol; Diabetes Mellitus, Experimental; Diabetic Retinopathy; Enzyme-Linked Immunosorbent Assay; Female; Gene Expression Regulation; Immunohistochemistry; Inflammation; Intercellular Adhesion Molecule-1; NF-kappa B; Oxidative Stress; Phosphodiesterase 3 Inhibitors; Rats; Rats, Wistar; Reactive Oxygen Species; Retina; Reverse Transcriptase Polymerase Chain Reaction; Streptozocin |
Appears in Collections: | 病理學科所 |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.