https://scholars.lib.ntu.edu.tw/handle/123456789/468859
Title: | CHC promotes tumor growth and angiogenesis through regulation of HIF-1α and VEGF signaling | Authors: | Tung K.-H. CHUNG-WU LIN Kuo C.-C. Li L.-T. Kuo Y.-H. Wu H.-C. |
Issue Date: | 2013 | Journal Volume: | 331 | Journal Issue: | 1 | Start page/Pages: | 58-67 | Source: | Cancer Letters | Abstract: | Pancreatic adenocarcinoma is an aggressive disease with a high mortality rate. In this study, we have newly generated a monoclonal antibody (mAb), Pa65-2, which specifically binds to pancreatic cancer cells and tumor blood vessels. The target protein of Pa65-2 is identified as human clathrin heavy chain (CHC). In vitro and In vivo study showed that suppression of CHC either by shRNA or by Pa65-2 inhibited tumor growth and angiogenesis. One of the key functions of CHC was to bind with the hypoxia-inducing factor (HIF)-1α protein, increasing the stability of this protein and facilitating its nuclear translocation, thereby regulating the expression of VEGF. Taken together, our findings indicate that CHC plays a role in the processes of tumorigenesis and angiogenesis. Pa65-2 antibody or CHC shRNA can potentially be used for pancreatic cancer therapy. ? 2012 Elsevier Ireland Ltd. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84873522077&doi=10.1016%2fj.canlet.2012.12.001&partnerID=40&md5=e5eeb838ee5900cba7a631d5de71124a https://scholars.lib.ntu.edu.tw/handle/123456789/468859 |
ISSN: | 0304-3835 | DOI: | 10.1016/j.canlet.2012.12.001 | SDG/Keyword: | clathrin heavy chain; gemcitabine; hypoxia inducible factor 1alpha; immunoglobulin G; monoclonal antibody; monoclonal antibody Pa65 2; short hairpin RNA; unclassified drug; vasculotropin; animal cell; animal experiment; animal model; animal tissue; antineoplastic activity; article; cancer growth; cancer inhibition; cancer therapy; carcinogenesis; controlled study; drug efficacy; drug protein binding; enzyme repression; human; human cell; human tissue; in vitro study; in vivo study; mouse; nonhuman; pancreas adenocarcinoma; pancreas cancer; priority journal; protein expression; protein function; protein protein interaction; protein stability; protein synthesis regulation; protein targeting; protein transport; signal transduction; Adenocarcinoma; Animals; Anoxia; Antibodies, Monoclonal; Apoptosis; Blotting, Western; Cell Cycle Proteins; Cell Proliferation; Chromatin Immunoprecipitation; Electrophoretic Mobility Shift Assay; Female; Fluorescent Antibody Technique; Gene Expression Regulation, Neoplastic; Guanine Nucleotide Exchange Factors; Humans; Hypoxia-Inducible Factor 1, alpha Subunit; Immunoenzyme Techniques; Immunoprecipitation; Mice; Mice, Inbred BALB C; Mice, Inbred NOD; Mice, SCID; Neovascularization, Pathologic; Nuclear Proteins; Pancreatic Neoplasms; Real-Time Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Signal Transduction; Tumor Cells, Cultured; Vascular Endothelial Growth Factor A |
Appears in Collections: | 病理學科所 |
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