https://scholars.lib.ntu.edu.tw/handle/123456789/469786
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Hsieh W.-J. | en_US |
dc.contributor.author | Liang C.-J. | en_US |
dc.contributor.author | Chieh J.-J. | en_US |
dc.contributor.author | SHU-HUEI WANG | en_US |
dc.contributor.author | I-RUE LAI | en_US |
dc.contributor.author | JYH-HORNG CHEN | en_US |
dc.contributor.author | FU-HSIUNG CHANG | en_US |
dc.contributor.author | Tseng W.-K. | en_US |
dc.contributor.author | Yang S.-Y. | en_US |
dc.contributor.author | CHAU-CHUNG WU | en_US |
dc.contributor.author | YUH-LIEN CHEN | en_US |
dc.creator | Chen Y.-L.;CHAU-CHUNG WU;Yang S.-Y.;Tseng W.-K.;Chang F.-H.;Chen J.-H.;Lai I.-R.;Wang S.-H.;Chieh J.-J.;Liang C.-J.;Hsieh W.-J. | - |
dc.date.accessioned | 2020-03-04T09:03:12Z | - |
dc.date.available | 2020-03-04T09:03:12Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 1176-9114 | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84870336445&doi=10.2147%2fIJN.S32154&partnerID=40&md5=2b5108e2999bcf37d9906ab505f53976 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/469786 | - |
dc.description.abstract | Background: Active targeting by specific antibodies combined with nanoparticles is a promising technology for cancer imaging and detection by magnetic resonance imaging (MRI). The aim of the present study is to investigate whether the systemic delivery of antivascular endothelial growth factor antibodies conjugating to the surface of functionalized supermagnetic iron oxide nanoparticles (anti-VEGF-NPs) led to target-specific accumulation in the tumor. Methods: The VEGF expression in human colon cancer and in Balb/c mice bearing colon cancers was examined by immunohistochemistry. The distribution of these anti-VEGF-NPs particles or NPs particles were evaluated by MRI at days 1, 2, or 9 after the injection into the jugular vein of Balb/c mice bearing colon cancers. Tumor and normal tissues (liver, spleen, lung, and kidney) were collected and were examined by Prussian blue staining to determine the presence and distribution of NPs in the tissue sections. Results: VEGF is highly expressed in human and mouse colon cancer tissues. MRI showed significant changes in the T*2 signal and T2 relaxation in the anti-VEGF-NP- injected-mice, but not in mice injected with NP alone. Examination of paraffin sections of tumor tissues stained for the iron constituent of the NPs with Prussian blue revealed a strong blue reaction in the tumors of anti-VEGF-NP-treated mice, but only a weak reaction in mice injected with NPs. In both groups, at all time points, Prussian blue-stained liver and spleen sections showed only light staining, while stained cells were rarely detected in kidney and lung sections. Transmission electron microscopy showed that many more electron-dense particles were present in endothelial cells, tumor cells, and extracellular matrix in tumor tissues in mice injected with anti-VEGF-NPs than in NP-injected mice. Conclusion: These results demonstrated in vivo tumor targeting and efficient accumulation of anti-VEGF-NPs in tumor tissues after systemic delivery in a colon cancer model, showing that anti-VEGF-NPs have potential for use as a molecular-targeted tumor imaging agent in vivo. ? 2012 Hsieh et al, publisher and licensee Dove Medical Press Ltd. | - |
dc.relation.ispartof | International Journal of Nanomedicine | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | antineoplastic agent; biological marker; dextran; ferric ferrocyanide; ultrasmall superparamagnetic iron oxide; unclassified drug; vascular endothelial growth factor antibody iron oxide nanoparticle conjugate; animal cell; animal experiment; animal tissue; article; cellular distribution; colon cancer; controlled study; disease marker; drug distribution; drug uptake; female; histopathology; immunocytochemistry; immunohistochemistry; immunoreactivity; in vitro study; in vivo study; male; material coating; molecular imaging; mouse; nonhuman; nuclear magnetic resonance imaging; particle size; protein determination; protein expression; protein localization; reaction time; tissue distribution; transmission electron microscopy; upregulation; Western blotting; Animals; Antibodies; Antineoplastic Agents; Cell Line, Tumor; Colonic Neoplasms; Dextrans; Female; Humans; Immunohistochemistry; Immunotoxins; Liver; Magnetic Resonance Imaging; Magnetite Nanoparticles; Male; Mice; Mice, Inbred BALB C; Microscopy, Electron, Transmission; Spleen; Vascular Endothelial Growth Factor A; Xenograft Model Antitumor Assays; Mus | - |
dc.title | In vivo tumor targeting and imaging with anti-vascular endothelial growth factor antibody-conjugated dextran-coated iron oxide nanoparticles | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.2147/IJN.S32154 | - |
dc.identifier.pmid | 22745546 | - |
dc.identifier.scopus | 2-s2.0-84870336445 | - |
dc.relation.pages | 2833 - 2842 | - |
dc.relation.journalvolume | 7 | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | none | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
crisitem.author.dept | Anatomy and Cell Biology | - |
crisitem.author.dept | Anatomy and Cell Biology | - |
crisitem.author.dept | Surgery-NTUH | - |
crisitem.author.dept | Surgery | - |
crisitem.author.dept | Biomedical Electronics and Bioinformatics | - |
crisitem.author.dept | Electrical Engineering | - |
crisitem.author.dept | Biomedical Engineering | - |
crisitem.author.dept | Center for Biotechnology | - |
crisitem.author.dept | Biochemistry and Molecular Biology | - |
crisitem.author.dept | Medical Education and Bioethics | - |
crisitem.author.dept | Internal Medicine-NTUH | - |
crisitem.author.dept | Anatomy and Cell Biology | - |
crisitem.author.orcid | 0000-0002-9208-3135 | - |
crisitem.author.orcid | 0000-0002-3444-8255 | - |
crisitem.author.orcid | 0000-0001-8191-475X | - |
crisitem.author.orcid | 0000-0002-6498-4008 | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Electrical Engineering and Computer Science | - |
crisitem.author.parentorg | College of Electrical Engineering and Computer Science | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Engineering | - |
crisitem.author.parentorg | Others: University-Level Research Centers | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
顯示於: | 醫學教育暨生醫倫理學科所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。