https://scholars.lib.ntu.edu.tw/handle/123456789/469984
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | WEN-MING HSU | en_US |
dc.contributor.author | Huang C.-C. | en_US |
dc.contributor.author | Lee H.-Y. | en_US |
dc.contributor.author | Wu P.-Y. | en_US |
dc.contributor.author | Wu M.-T. | en_US |
dc.contributor.author | Chuang H.-C. | en_US |
dc.contributor.author | Lin L.-L. | en_US |
dc.contributor.author | Chuang J.-H. | en_US |
dc.creator | Wen-Ming Hsu;Huang C.-C.;Lee H.-Y.;Wu P.-Y.;Wu M.-T.;Chuang H.-C.;Lin L.-L.;Chuang J.-H. | - |
dc.date.accessioned | 2020-03-05T03:20:12Z | - |
dc.date.available | 2020-03-05T03:20:12Z | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 1949-2553 | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84944472825&doi=10.18632%2foncotarget.4511&partnerID=40&md5=5072b3c639c2c3405b585117b262fcaf | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/469984 | - |
dc.description.abstract | Toll-like receptor3 (TLR3) has been confirmed to be differentially expressed in neuroblastoma (NB), and predicts a favorable prognosis with a high expression in tumor tissues. Treatment with TLR3 agonist - polyinosinic-polycytidylic acid [poly(I:C)] could induce significant but limited apoptosis in TLR3-expressing NB cells, suggesting that other viral RNA sensors, including melanoma differentiationassociated gene 5 (MDA5) and retinoic acid-inducible gene-I (RIG-I) in the cytosolic compartment might also be implicated in poly(I:C)-induced NB cell death. MDA5 and RIG-I were induced by poly(I:C) to express in two of six NB cell lines, SK-NAS (AS) and SK-N-FI, which were associated with up-regulation of caspase9 and active caspase3. While knockdown of either MDA5 or RIG-I alone is ineffective to decrease caspase9 and active caspase3, simultaneously targeting MDA5 and TLR3 showed the best effect to rescue poly(I:C) induced up-regulation of mitochondrial antiviral signaling protein (MAVS), caspase9, active caspase3, and apoptosis in AS cells. Over-expression of MDA5 in FaDu cells resulted in significantly less colony formation and more poly(I:C)-induced cell death. Further studies in human NB tissue samples revealed that MDA5 expression in NB tissues predicted a favorable prognosis synergistically with TLR3. Our findings indicate that MDA5 may serve as a complementary role in the TLR3 activated suppression of NB. | - |
dc.publisher | Impact Journals LLC | - |
dc.relation.ispartof | Oncotarget | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | caspase 3; caspase 9; melanoma differentiation gene 5; messenger RNA; mitochondrial antiviral signaling protein; oncoprotein; polyinosinic polycytidylic acid; reactive oxygen metabolite; retinoic acid inducible protein I; small interfering RNA; toll like receptor 3; unclassified drug; caspase 3; caspase 9; DDX58 protein, human; DEAD box protein; IFIH1 protein, human; interferon induced helicase C domain containing protein 1; polyinosinic polycytidylic acid; reactive oxygen metabolite; retinoic acid inducible protein I; TLR3 protein, human; toll like receptor 3; apoptosis; Article; cancer inhibition; controlled study; drug targeting; enzyme activation; gene expression regulation; gene overexpression; gene silencing; gene targeting; human; human cell; MAVS gene; MDA5 gene; neuroblastoma; neuroblastoma cell line; protein expression; RIG I gene; TLR3 gene; agonists; antagonists and inhibitors; child; drug effects; female; genetics; infant; Kaplan Meier method; male; metabolism; mitochondrion; neuroblastoma; newborn; pathology; preschool child; reverse transcription polymerase chain reaction; RNA interference; Western blotting; Apoptosis; Blotting, Western; Caspase 3; Caspase 9; Child; Child, Preschool; DEAD Box Protein 58; DEAD-box RNA Helicases; Female; Gene Expression Regulation, Neoplastic; Humans; Infant; Infant, Newborn; Interferon-Induced Helicase, IFIH1; Kaplan-Meier Estimate; Male; Mitochondria; Neuroblastoma; Poly I-C; Reactive Oxygen Species; Reverse Transcriptase Polymerase Chain Reaction; RNA Interference; Toll-Like Receptor 3 | - |
dc.title | MDA5 complements TLR3 in suppression of neuroblastoma | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.18632/oncotarget.4511 | - |
dc.identifier.pmid | 26208481 | - |
dc.identifier.scopus | 2-s2.0-84944472825 | - |
dc.relation.pages | 24935-24946 | - |
dc.relation.journalvolume | 6 | - |
dc.relation.journalissue | 28 | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | none | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
crisitem.author.dept | Surgery-NTUH | - |
crisitem.author.dept | Surgery | - |
crisitem.author.orcid | 0000-0002-5145-9538 | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
顯示於: | 醫學系 |
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