https://scholars.lib.ntu.edu.tw/handle/123456789/473301
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Li Y.-T. | en_US |
dc.contributor.author | CHUN-JEN LIU | en_US |
dc.contributor.author | TUNG-HUNG SU | en_US |
dc.contributor.author | Cheng H.-R. | en_US |
dc.contributor.author | YUNG-MING JENG | en_US |
dc.contributor.author | Lin H.-L. | en_US |
dc.contributor.author | Wang C.-C. | en_US |
dc.contributor.author | JIA-HORNG KAO | en_US |
dc.contributor.author | PEI-JER CHEN | en_US |
dc.contributor.author | DING-SHINN CHEN | en_US |
dc.contributor.author | Wu H.-L. | en_US |
dc.creator | Li Y.-T.;Liu C.-J.;Su T.-H.;Cheng H.-R.;Yung-Ming Jeng;Lin H.-L.;Wang C.-C.;Kao J.-H.;Chen P.-J.;Chen D.-S.;Wu H.-L. | - |
dc.date.accessioned | 2020-03-06T08:24:57Z | - |
dc.date.available | 2020-03-06T08:24:57Z | - |
dc.date.issued | 2016 | - |
dc.identifier.issn | 1949-2553 | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84984550357&doi=10.18632%2foncotarget.9986&partnerID=40&md5=89cf4a328fb9628060b24b5dfeafc771 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/473301 | - |
dc.description.abstract | The metastatic tumor antigen 1 (MTA1) protein is associated with tumor invasiveness and poor prognosis in patients with hepatocellular carcinoma (HCC), particularly in those with hepatitis B virus (HBV)-related HCC. Chronically woodchuck hepatitis virus (WHV)-infected woodchuck is an ideal animal model for studying the pathogenesis of HBV-associated liver diseases, including HCC. To investigate the roles of MTA1 in HBV-associated hepatocarcinogenesis in the woodchuck model, we cloned the woodchuck MTA1 (wk-MTA1) complementary (c)DNA and characterized its molecular functions. The sequence and organization of the wk-MTA1 protein were highly conserved among different species. Similar to its expression in human HCC, wk-MTA1 was upregulated in woodchuck HCC, as determined at RNA and protein levels. Furthermore, an MTA1-spliced variant, wk-MTA1dE4, was overexpressed in woodchuck HCC, and it was attributed to approximately 50% of the total transcripts. The percentage of wk-MTA1dE4-overexpressed woodchuck HCCs was higher than that of the total wk-MTA1-overexpressed HCCs (77.8% vs 61.1%) and wk-MTA1dE4 may represent a more sensitive marker than the total wk-MTA1 in woodchuck HCC. We overexpressed or knocked down wk-MTA1 in a woodchuck HCC cell line and demonstrated that wk-MTA1 could interact with the WHV X protein (WHx) and play indispensable roles in WHx-mediated NF-κB activation and tumor cell migration- and invasion-promoting activities. In conclusion, our results support the hypothesis that woodchuck HCC recapitulates HBV-associated HCC with respect to the molecular characteristics of MTA1 and provides new clues for conducting mechanistic studies of MTA1 in HBV-associated hepatocarcinogenesis, including the possible clinical significance of wk-MTA1dE4. | en_US |
dc.publisher | Impact Journals LLC | en_US |
dc.relation.ispartof | Oncotarget | en_US |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | complementary DNA; hepatitis B virus X protein; immunoglobulin enhancer binding protein; metastatic tumor antigen 1; RNA; tumor antigen; unclassified drug; hepatitis B virus X protein; immunoglobulin enhancer binding protein; transactivator protein; tumor antigen; amino acid sequence; animal experiment; animal model; animal tissue; Article; cancer cell culture; cancer growth; cell invasion; cell migration; controlled study; genetic transfection; Hepatitis B virus; hepatocellular carcinoma cell line; immunohistochemistry; liver; liver carcinogenesis; liver cell carcinoma; molecular cloning; nonhuman; Northern blotting; plasmid; protein analysis; protein interaction; reverse transcription polymerase chain reaction; RNA extraction; sequence analysis; upregulation; woodchuck; animal; disease exacerbation; disease model; female; genetics; human; liver cell carcinoma; liver tumor; Marmota; metabolism; pathology; signal transduction; Amino Acid Sequence; Animals; Antigens, Neoplasm; Carcinoma, Hepatocellular; Disease Models, Animal; Disease Progression; Female; Humans; Liver Neoplasms; Marmota; NF-kappa B; Signal Transduction; Trans-Activators; Transfection | - |
dc.title | Characterization of metastatic tumor antigen 1 and its interaction with hepatitis B virus X protein in NF-κB signaling and tumor progression in a woodchuck hepatocellular carcinoma model | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.18632/oncotarget.9986 | - |
dc.identifier.pmid | 27323415 | - |
dc.identifier.scopus | 2-s2.0-84984550357 | - |
dc.relation.pages | 47173-47185 | en_US |
dc.relation.journalvolume | 7 | en_US |
dc.relation.journalissue | 30 | en_US |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
crisitem.author.dept | Internal Medicine-NTUH | - |
crisitem.author.dept | Internal Medicine | - |
crisitem.author.dept | Internal Medicine-NTUH | - |
crisitem.author.dept | Pathology | - |
crisitem.author.dept | Pathology-NTUH | - |
crisitem.author.dept | Clinical Medicine | - |
crisitem.author.dept | Internal Medicine-NTUH | - |
crisitem.author.dept | Clinical Medicine | - |
crisitem.author.dept | Internal Medicine-NTUH | - |
crisitem.author.orcid | 0000-0002-6202-0993 | - |
crisitem.author.orcid | 0000-0002-6747-7941 | - |
crisitem.author.orcid | 0000-0002-3878-4491 | - |
crisitem.author.orcid | 0000-0002-2442-7952 | - |
crisitem.author.orcid | 0000-0001-8316-3785 | - |
crisitem.author.orcid | 0000-0001-7791-6154 | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
顯示於: | 病理學科所 |
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