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  1. NTU Scholars
  2. 醫學院
  3. 病理學科所
Please use this identifier to cite or link to this item: https://scholars.lib.ntu.edu.tw/handle/123456789/473380
Title: Prognostic significance of TWEAK expression in colorectal cancer and effect of its inhibition on invasion
Authors: Lin B.-R.
Huang M.-T.
Chen S.-T.
YUNG-MING JENG 
Li Y.-J.
Liang J.-T.
Lee P.-H.
Chang K.-J.
Chang C.-C.
Issue Date: 2012
Journal Volume: 19
Journal Issue: SUPPL. 3
Start page/Pages: S385-S394
Source: Annals of Surgical Oncology
Abstract: 
Background: Tumor necrosis factor-like weak inducer of apoptosis (TWEAK) has been implicated in tumor development and progression. The aim of this study was to investigate the role of TWEAK in colorectal cancer (CRC) progression. Methods: To investigate the involvement of TWEAK in the progression of human CRC, normal, and tumor specimens from 174 patients were analyzed immunohistochemically for the expression of TWEAK. TWEAK recombinant protein treatment, transfection of expression plasmids, and small interfering RNA to knockdown TWEAK expression were performed to test invasive ability with a Boyden chamber. The mRNA expression profile in recombinant TWEAK treatment was compared to a control group by microarray analysis. To identify downstream effectors, Raf kinase inhibitor (RKIP) and its correlation with TWEAK in vitro and in vivo were examined by quantitative real-time polymerase chain reaction and invasion assays. Results: CRC patients whose tumors displayed high TWEAK expression had a statistically significantly higher overall survival and a disease-free advantage over those with a low TWEAK expression. In in vitro invasion assays, alterations in TWEAK expression in CRC cell lines inversely modulated their invasive ability. By means of integrated genomics, we identified RKIP as a downstream effector in TWEAK-mediated invasion inhibition. Knockout of RKIP expression in HCT116 cells by short hairpin RNA (shRKIP) resulted in increased invasiveness. Clinically, RKIP and TWEAK mRNA expression showed strong positive correlations in CRC patient samples. Conclusions: Our results implicate TWEAK as a key regulator of CRC invasion, and it appears to be a useful prognostic factor for patients with CRC. ? 2011 Society of Surgical Oncology.
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84865003622&doi=10.1245%2fs10434-011-1825-x&partnerID=40&md5=62fd06a38a507e2216d0b2b18aa8b8eb
https://scholars.lib.ntu.edu.tw/handle/123456789/473380
ISSN: 1068-9265
DOI: 10.1245/s10434-011-1825-x
metadata.dc.subject.other: B Raf kinase inhibitor; cytokine; messenger RNA; recombinant protein; short hairpin RNA; small interfering RNA; tumor necrosis factor like weak inducer of apoptosis; unclassified drug; adult; aged; article; cancer cell culture; cancer growth; cancer invasion; cell strain HCT116; colorectal cancer; control group; female; genome; human; human tissue; immunohistochemistry; in vitro study; in vivo study; major clinical study; male; microarray analysis; overall survival; plasmid; prognosis; protein expression; reverse transcription polymerase chain reaction; Aged; Colorectal Neoplasms; Disease-Free Survival; Down-Regulation; Female; Gene Knockout Techniques; HCT116 Cells; HT29 Cells; Humans; Intestinal Mucosa; Kaplan-Meier Estimate; Lymphatic Metastasis; Male; Middle Aged; Multivariate Analysis; Neoplasm Invasiveness; Neoplasm Recurrence, Local; Oligonucleotide Array Sequence Analysis; Phosphatidylethanolamine Binding Protein; Plasmids; Predictive Value of Tests; Proportional Hazards Models; Transfection; Tumor Markers, Biological; Tumor Necrosis Factors; Up-Regulation
[SDGs]SDG3
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臺大位居世界頂尖大學之列,為永久珍藏及向國際展現本校豐碩的研究成果及學術能量,圖書館整合機構典藏(NTUR)與學術庫(AH)不同功能平台,成為臺大學術典藏NTU scholars。期能整合研究能量、促進交流合作、保存學術產出、推廣研究成果。

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