https://scholars.lib.ntu.edu.tw/handle/123456789/476219
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | SHENG-HONG TSENG | en_US |
dc.contributor.author | Hwang L.-H. | en_US |
dc.contributor.author | Lin S.-M. | en_US |
dc.creator | Sheng-Hong Tseng;Hwang L.-H.;Lin S.-M. | - |
dc.date.accessioned | 2020-03-12T08:24:52Z | - |
dc.date.available | 2020-03-12T08:24:52Z | - |
dc.date.issued | 1997 | - |
dc.identifier.issn | 1524-9557 | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0031434207&doi=10.1097%2f00002371-199709000-00002&partnerID=40&md5=a385a8a5d799760b1b99903d271997c7 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/476219 | - |
dc.description.abstract | To test whether cytokine gene therapy can be applied to an immunologically privileged site, such as the brain, we investigated antitumor immunity in the brain induced by cytokine-secreting glioma cells. Three cytokine genes, interleukin-2 (IL-2), interleukin-4 (IL-4), and granulocyte-macrophage colony-stimulating factor (GM-CSF) were transduced into a rat C6 glioma cell line via a retroviral vector, S2. Rats intracerebrally (IC) implanted with the C6 cells genetically engineered to secrete the cytokines, especially GM-CSF, manifested significantly higher survival rates than those with C6 cells or with C6 cells bearing the control vector (p < 0.002). In vivo, C6 tumors bearing the cytokine genes grew more slowly than wild-type tumors at any time point, and eventually diminished within 6 weeks after tumor cell implantation. Histopathological and immunohistochemical studies revealed that different cytokines induced diverse immune reactions. In the IL-2 group, CD4+ and CD8+ T cells dominated from day 3 to week 4, but disappeared at week 6. Some granulocytes were noted between weeks 2 and 4. In the IL-4 group, eosinophils were noted from day 3 to week 4, and CD4+ and CD8+ T cells, as well as macrophages at week 2. At week 6, only residual levels of macrophages and CD8+ T cells remained. In the GM-CSF group, granulocytes appeared as early as day 1 post-IC tumor implantation, and macrophages at day 2. CD4+ and CD8+ T cells were found from day 3 to week 4. At week 6, only residual CD4+ T cells and macrophages remained. Long-lasting antitumor immunity was confirmed in all groups by rechallenging surviving rats with wild-type C6 cells in the brain 100 days after implanting cytokine gene-bearing C6 cells. In vivo depletion of GM-CSF by anti-GM-CSF antibody further confirmed that the immune reaction induced by GM-CSF-secreting tumor cells were mainly from the action of GM-CSF, rather than the immunogenicity of C6 cells. ? 1997 Lippincott-Raven Publishers. | - |
dc.relation.ispartof | Journal of Immunotherapy | - |
dc.subject | Glioma; GM-CSF; IL-2; IL-4; Tumorigenicity | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | cytokine; granulocyte macrophage colony stimulating factor; interleukin 2; interleukin 4; virus vector; animal cell; animal experiment; animal model; article; cancer transplantation; controlled study; cytotoxic T lymphocyte; eosinophil; gene therapy; genetic engineering; genetic transduction; glioma cell; helper cell; immunohistochemistry; immunotherapy; macrophage; nonhuman; priority journal; rat; tumor immunity | - |
dc.title | Induction of antitumor immunity by intracerebrally implanted rat c6 glioma cells genetically engineered to secrete cytokines | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1097/00002371-199709000-00002 | - |
dc.identifier.pmid | 9336740 | - |
dc.identifier.scopus | 2-s2.0-0031434207 | - |
dc.relation.pages | 334-342 | - |
dc.relation.journalvolume | 20 | - |
dc.relation.journalissue | 5 | - |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.fulltext | no fulltext | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
crisitem.author.dept | Surgery-NTUH | - |
crisitem.author.orcid | 0000-0002-9959-9206 | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
顯示於: | 醫學系 |
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