https://scholars.lib.ntu.edu.tw/handle/123456789/480430
標題: | Evaluation of antibody responses against SARS coronaviral nucleocapsid or spike proteins by immunoblotting or ELISA | 作者: | Huang L.-R. Chiu C.-M. Shiou-Hwei Yeh Huang W.-H. PO-REN HSUEH Yang W.-Z. Yang J.-Y. Su I.-J. SHAN-CHWEN CHANG PEI-JER CHEN |
公開日期: | 2004 | 卷: | 73 | 期: | 3 | 起(迄)頁: | 338-346 | 來源出版物: | Journal of Medical Virology | 摘要: | Severe acute respiratory syndrome (SARS)-CoV is a newly emerging virus that causes SARS with high mortality rate in infected people. To study the humoral responses against SARS-CoV, we evaluated nucleocapsid (N) and spike (S) proteins-specific antibodies in patients' sera by Western blotting and enzyme-linked immunosorbent assay (ELISA). Recombinant N and S proteins of SARS-CoV were purified from transformed E. coli. Serum specimens from 40 SARS-CoV-infected patients in the convalescent phase were analyzed by Western blotting using the purified antigens. Serial serum specimens from 12 RT-PCR-confirmed SARS patients were assayed by ELISA using the recombinant N protein as coated antigen. By Western blotting, 97.5% of the SARS patients were positive for N protein-specific antibodies whereas only 47.5% of the samples were positive for S protein-specific antibodies. Using N protein-based ELISA, 10 out of the 12 patients were positive for N protein-specific antibodies and 6 of them showed seroconversion at mean of 16 days after onset of fever. Immunoblotting was useful for detecting the humoral immune response after SARS-CoV infection. Antibodies against SARS-CoV N protein appear at the early stage of infection, therefore, N protein-based ELISA could serve as a simple, sensitive, and specific test for diagnosing SARS-CoV infection. ? 2004 Wiley-Liss, Inc. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/480430 | ISSN: | 0146-6615 | DOI: | 10.1002/jmv.20096 | SDG/關鍵字: | nucleocapsid protein; recombinant protein; virus antibody; virus antigen; virus spike protein; antibody detection; antibody response; antibody specificity; article; clinical article; diagnostic test; enzyme linked immunosorbent assay; Escherichia coli; human; humoral immunity; immunoblotting; nonhuman; nucleotide sequence; protein purification; reverse transcription polymerase chain reaction; SARS coronavirus; seroconversion; severe acute respiratory syndrome; Western blotting; SARS CoV |
顯示於: | 醫學院附設醫院 (臺大醫院) |
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