https://scholars.lib.ntu.edu.tw/handle/123456789/480430
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Huang L.-R. | en_US |
dc.contributor.author | Chiu C.-M. | en_US |
dc.contributor.author | Shiou-Hwei Yeh | en_US |
dc.contributor.author | Huang W.-H. | en_US |
dc.contributor.author | PO-REN HSUEH | en_US |
dc.contributor.author | Yang W.-Z. | en_US |
dc.contributor.author | Yang J.-Y. | en_US |
dc.contributor.author | Su I.-J. | en_US |
dc.contributor.author | SHAN-CHWEN CHANG | en_US |
dc.contributor.author | PEI-JER CHEN | en_US |
dc.creator | Chen P.-J.;Chang S.-C.;Su I.-J.;Yang J.-Y.;Yang W.-Z.;PO-REN HSUEH;Huang W.-H.;Yeh S.-H.;Chiu C.-M.;Huang L.-R. | - |
dc.date.accessioned | 2020-03-27T05:30:59Z | - |
dc.date.available | 2020-03-27T05:30:59Z | - |
dc.date.issued | 2004 | - |
dc.identifier.issn | 0146-6615 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/480430 | - |
dc.description.abstract | Severe acute respiratory syndrome (SARS)-CoV is a newly emerging virus that causes SARS with high mortality rate in infected people. To study the humoral responses against SARS-CoV, we evaluated nucleocapsid (N) and spike (S) proteins-specific antibodies in patients' sera by Western blotting and enzyme-linked immunosorbent assay (ELISA). Recombinant N and S proteins of SARS-CoV were purified from transformed E. coli. Serum specimens from 40 SARS-CoV-infected patients in the convalescent phase were analyzed by Western blotting using the purified antigens. Serial serum specimens from 12 RT-PCR-confirmed SARS patients were assayed by ELISA using the recombinant N protein as coated antigen. By Western blotting, 97.5% of the SARS patients were positive for N protein-specific antibodies whereas only 47.5% of the samples were positive for S protein-specific antibodies. Using N protein-based ELISA, 10 out of the 12 patients were positive for N protein-specific antibodies and 6 of them showed seroconversion at mean of 16 days after onset of fever. Immunoblotting was useful for detecting the humoral immune response after SARS-CoV infection. Antibodies against SARS-CoV N protein appear at the early stage of infection, therefore, N protein-based ELISA could serve as a simple, sensitive, and specific test for diagnosing SARS-CoV infection. ? 2004 Wiley-Liss, Inc. | en_US |
dc.relation.ispartof | Journal of Medical Virology | en_US |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | nucleocapsid protein; recombinant protein; virus antibody; virus antigen; virus spike protein; antibody detection; antibody response; antibody specificity; article; clinical article; diagnostic test; enzyme linked immunosorbent assay; Escherichia coli; human; humoral immunity; immunoblotting; nonhuman; nucleotide sequence; protein purification; reverse transcription polymerase chain reaction; SARS coronavirus; seroconversion; severe acute respiratory syndrome; Western blotting; SARS CoV | - |
dc.title | Evaluation of antibody responses against SARS coronaviral nucleocapsid or spike proteins by immunoblotting or ELISA | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1002/jmv.20096 | - |
dc.identifier.pmid | 15170626 | - |
dc.identifier.scopus | 2-s2.0-84984533509 | - |
dc.relation.pages | 338-346 | en_US |
dc.relation.journalvolume | 73 | en_US |
dc.relation.journalissue | 3 | en_US |
item.cerifentitytype | Publications | - |
item.grantfulltext | none | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
crisitem.author.dept | Microbiology | - |
crisitem.author.dept | Laboratory Medicine | - |
crisitem.author.dept | Laboratory Medicine-NTUH | - |
crisitem.author.dept | Internal Medicine | - |
crisitem.author.dept | Internal Medicine-NTUH | - |
crisitem.author.dept | Office of the Vice President | - |
crisitem.author.dept | Clinical Pharmacy | - |
crisitem.author.dept | Medical Education and Bioethics | - |
crisitem.author.dept | Clinical Medicine | - |
crisitem.author.orcid | 0000-0003-4509-180X | - |
crisitem.author.orcid | 0000-0002-7502-9225 | - |
crisitem.author.orcid | 0000-0001-6505-4139 | - |
crisitem.author.orcid | 0000-0001-8316-3785 | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | Administrative Unit | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
顯示於: | 醫學院附設醫院 (臺大醫院) |
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