https://scholars.lib.ntu.edu.tw/handle/123456789/484558
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Huang, R.-X. | en_US |
dc.contributor.author | Fu, Y. | en_US |
dc.contributor.author | Liu, W. | en_US |
dc.contributor.author | Ma, Y.-T. | en_US |
dc.contributor.author | Hsieh, B.-Y. | en_US |
dc.contributor.author | SHU-CHING CHEN | en_US |
dc.contributor.author | Sun, M. | en_US |
dc.contributor.author | Li, P.-C. | en_US |
dc.contributor.author | PAI-CHI LI | zz |
dc.creator | Huang, R.-X.;Fu, Y.;Liu, W.;Ma, Y.-T.;Hsieh, B.-Y.;Chen, S.-C.;Sun, M.;Li, P.-C. | - |
dc.date.accessioned | 2020-04-16T02:35:34Z | - |
dc.date.available | 2020-04-16T02:35:34Z | - |
dc.date.issued | 2018 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/484558 | - |
dc.description.abstract | Monitoring dynamic interactions of T cells migrating toward tumor is beneficial to understand how cancer immunotherapy works. Optical-resolution photoacoustic microscope (OR-PAM) can provide not only high spatial resolution but also deeper penetration than conventional optical microscopy. With the aid of exogenous contrast agents, the dual-wavelength OR-PAM can be applied to map the distribution of CD8+ cytotoxic T lymphocytes (CTLs) with gold nanospheres (AuNS) under 523nm laser irradiation and Hepta1-6 tumor spheres with indocyanine green (ICG) under 800nm irradiation. However, at 1K laser PRF, it takes approximately 20 minutes to obtain a full sample volume of 160 × 160 × 150 μm3. To increase the imaging rate, we propose a random non-uniform sparse sampling mechanism to achieve fast sparse photoacoustic data acquisition. The image recovery process is formulated as a low-rank matrix recovery (LRMR) based on compressed sensing (CS) theory. We show that it could be stably recovered via nuclear-norm minimization optimization problem to maintain image quality from a significantly fewer measurement. In this study, we use the dual-wavelength OR-PAM with CS to visualize T cell trafficking in a 3D culture system with higher temporal resolution. Data acquisition time is reduced by 40% in such sample volume where sampling density is 0.5. The imaging system reveals the potential to understand the dynamic cellular process for preclinical screening of anti-cancer drugs. ? COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only. | - |
dc.relation.ispartof | Progress in Biomedical Optics and Imaging - Proceedings of SPIE | - |
dc.subject | 3D Cell Culture; Compressed Sensing; Matrix Completion; Photoacoustic Microscopy; Sparse Sampling | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | Cell culture; Compressed sensing; Data acquisition; Diagnosis; Diseases; Gold compounds; Image processing; Irradiation; Matrix algebra; Optical systems; Photons; T-cells; Tumors; 3-D cell culture; Cytotoxic T lymphocytes; High spatial resolution; Low-rank matrix recoveries; Matrix completion; Nuclear norm minimizations; Optimization problems; Sparse sampling; Photoacoustic microscopy | - |
dc.title | Dual-wavelength OR-PAM with compressed sensing for cell tracking in a 3D cell culture system | en_US |
dc.type | conference paper | en |
dc.identifier.doi | 10.1117/12.2289975 | - |
dc.identifier.scopus | 2-s2.0-85047366525 | - |
dc.identifier.url | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85047366525&doi=10.1117%2f12.2289975&partnerID=40&md5=080be476263860556739452c7bc804ed | - |
dc.relation.journalvolume | 10494 | - |
item.openairetype | conference paper | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_5794 | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
crisitem.author.dept | Internal Medicine-NTUH | - |
crisitem.author.dept | Biomedical Electronics and Bioinformatics | - |
crisitem.author.dept | Electrical Engineering | - |
crisitem.author.orcid | 0000-0001-9112-1526 | - |
crisitem.author.orcid | 0000-0001-7603-7852 | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Electrical Engineering and Computer Science | - |
crisitem.author.parentorg | College of Electrical Engineering and Computer Science | - |
顯示於: | 生醫電子與資訊學研究所 |
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