https://scholars.lib.ntu.edu.tw/handle/123456789/485849
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Kuo, C.-T. | en_US |
dc.contributor.author | Wang, J.-Y. | en_US |
dc.contributor.author | Lin, Y.-F. | en_US |
dc.contributor.author | Wo, A.M. | en_US |
dc.contributor.author | Chen, B.P.C. | en_US |
dc.contributor.author | ANDREW WO | en_US |
dc.contributor.author | H. Lee | en_US |
dc.creator | ANDREW WO;Lee, H.;Chen, B.P.C.;Wo, A.M.;Lin, Y.-F.;Wang, J.-Y.;Kuo, C.-T. | - |
dc.date.accessioned | 2020-04-28T07:11:09Z | - |
dc.date.available | 2020-04-28T07:11:09Z | - |
dc.date.issued | 2017 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/485849 | - |
dc.description.abstract | Biomaterial-based tissue culture platforms have emerged as useful tools to mimic in vivo physiological microenvironments in experimental cell biology and clinical studies. We describe herein a three-dimensional (3D) tissue culture platform using a polydimethylsiloxane (PDMS)-based hanging drop array (PDMS-HDA) methodology. Multicellular spheroids can be achieved within 24 h and further boosted by incorporating collagen fibrils in PDMS-HDA. In addition, the spheroids generated from different human tumor cells exhibited distinct sensitivities toward drug chemotherapeutic agents and radiation as compared with two-dimensional (2D) cultures that often lack in vivo-like biological insights. We also demonstrated that multicellular spheroids may enable key hallmarks of tissue-based bioassays, including drug screening, tumor dissemination, cell co-culture, and tumor invasion. Taken together, these results offer new opportunities not only to achieve the active control of 3D multicellular spheroids on demand, but also to establish a rapid and cost-effective platform to study anti-cancer therapeutics and tumor microenvironments. ? 2017 The Author(s). | - |
dc.relation.ispartof | Scientific Reports | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | biocompatible coated material; dimeticone; bioassay; cell culture technique; coculture; human; multicellular spheroid; preclinical study; procedures; tumor cell line; Biological Assay; Cell Culture Techniques; Cell Line, Tumor; Coated Materials, Biocompatible; Coculture Techniques; Dimethylpolysiloxanes; Drug Evaluation, Preclinical; Humans; Spheroids, Cellular | - |
dc.title | Three-dimensional spheroid culture targeting versatile tissue bioassays using a PDMS-based hanging drop array | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1038/s41598-017-04718-1 | - |
dc.identifier.scopus | 2-s2.0-85021651388 | - |
dc.identifier.url | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85021651388&doi=10.1038%2fs41598-017-04718-1&partnerID=40&md5=cd8ffde01f4490bc03a7f3c100b724cd | - |
dc.relation.journalvolume | 7 | - |
dc.relation.journalissue | 1 | - |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.fulltext | no fulltext | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
crisitem.author.dept | Applied Mechanics | - |
crisitem.author.dept | Biomedical Electronics and Bioinformatics | - |
crisitem.author.dept | Life Science | - |
crisitem.author.dept | Center for Biotechnology | - |
crisitem.author.dept | Electrical Engineering | - |
crisitem.author.orcid | 0000-0002-1477-0183 | - |
crisitem.author.parentorg | College of Engineering | - |
crisitem.author.parentorg | College of Electrical Engineering and Computer Science | - |
crisitem.author.parentorg | College of Life Science | - |
crisitem.author.parentorg | Others: University-Level Research Centers | - |
crisitem.author.parentorg | College of Electrical Engineering and Computer Science | - |
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