https://scholars.lib.ntu.edu.tw/handle/123456789/486128
標題: | Capturing CD4 cells using a functionalized circular microfluidic device and glutaraldehyde as biolinker for tuberculosis detection and diagnosis | 作者: | Shih, Y.-F. Huang, N.-T. NIEN-TSU HUANG CHIH-KUNG LEE |
公開日期: | 2015 | 卷: | 9320 | 來源出版物: | Progress in Biomedical Optics and Imaging - Proceedings of SPIE | 摘要: | It is estimated that about one-third of the world?€?s population has already been infected by tuberculosis. Mycobacterium tuberculosis, in general, can result in an active case of tuberculosis in approximately 5%-10% of those who suffer from latent tuberculosis and the chance of becoming ill is the highest within one of year of getting the disease. Although a newly developed methods called interferon gamma release assay (IGRA) can monitor CD4 cells secreted cytokine to diagnose tuberculosis (TB) condition. However, it is difficult to count total numbers of cytokine secreted CD4 cells, which make the diagnosis less accurate. Therefore, we develop a functionalized polydimethylsiloxane (PDMS) device using glutaraldehyde to capture CD4 cells. To enhance the capture efficiency, we use COMSOL simulation to optimize the arrangement of PDMS micro pillars to make cells uniformly distributed in the device. Our preliminary data showed the microfluidic configuration in a circular shape with HCP patterned micro pillars turned 30 degrees offers the highest cell capture rate. ? 2015 SPIE. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/486128 | DOI: | 10.1117/12.2078693 | SDG/關鍵字: | BioMEMS; Cells; Cytology; Diagnosis; Microchannels; Microsystems; Silicones; Tubes (components); Capture efficiency; Cell capture; Detection and diagnosis; Interferon-gamma; Micro-fluidic devices; Mycobacterium tuberculosis; Polydimethylsiloxane PDMS; tuberculosis; Microfluidics |
顯示於: | 應用力學研究所 |
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