https://scholars.lib.ntu.edu.tw/handle/123456789/490859
Title: | A novel DNA selection and direct extraction process and its application in DNA recombination | Authors: | AN-BANG WANG Cheng C.-W. Lin I.-C. Lu F.-Y. Tsai H.-J. Lin C.-C. Yang C.-H. Pan P.-T. Kuan C.-C. Chen Y.-C. Lin Y.-W. Chang C.-N. Wu Y.-H. Kurniawan T. CHII-WANN LIN ANDREW WO LIN-CHI CHEN |
Keywords: | Chip integration; DNA recombination; DNA selection and extraction; Microfluidics | Issue Date: | 2011 | Journal Volume: | 32 | Journal Issue: | 3-4 | Start page/Pages: | 423-430 | Source: | Electrophoresis | Abstract: | In the conventional bench-top approach, the DNA recombination process is time- and effort-consuming due to laborious procedures lasting from several hours to a day. A novel DNA selection and direct extraction process has been proposed, integrated and tested on chip. The integrative microfluidic chip can perform the whole procedure of DNA recombination, including DNA digestion, gel electrophoresis, DNA extraction and insert-vector ligation within 1h. In this high-throughput design, the manual gel cutting was replaced by an automatic processing system that performed high-quality and high-recovery efficiency in DNA extraction process. With no need of gel-dissolving reagents and manipulation, the application of selection and direct extraction process could significantly eliminate the risks from UV and EtBr and also facilitate DNA recombination. Reliable output with high success rate of cloning has been achieved with a significant reduction in operational hazards, required materials, efforts and time. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-79551641343&doi=10.1002%2felps.201000449&partnerID=40&md5=8952f62e8a5bd3c6ba114d16a1277573 https://scholars.lib.ntu.edu.tw/handle/123456789/490859 |
ISSN: | 01730835 | DOI: | 10.1002/elps.201000449 | SDG/Keyword: | ethidium bromide; article; automation; DNA degradation; DNA determination; DNA extraction; DNA recombination; gel electrophoresis; microfluidics; risk management; ultraviolet radiation; DNA; Electrophoresis; Equipment Design; Equipment Failure Analysis; Microfluidic Analytical Techniques; Microfluidics; Oligonucleotide Array Sequence Analysis; Recombination, Genetic |
Appears in Collections: | 應用力學研究所 |
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