https://scholars.lib.ntu.edu.tw/handle/123456789/495779
Title: | The role of nocturnin in early adipogenesis and modulation of systemic insulin resistance in human | Authors: | Hee S.-W. Tsai S.-H. YI-CHENG CHANG Chang C.-J. Yu I.-S. PO-CHU LEE Lee W.-J. Yun-Chia Chang E. LEE-MING CHUANG |
Issue Date: | 2012 | Journal Volume: | 20 | Journal Issue: | 8 | Start page/Pages: | 1558-1565 | Source: | Obesity | Abstract: | The deadenylase nocturnin (Noc, Ccrn4l) has been recently found to regulate lipid metabolism and to control preadipocyte differentiation. Here, we showed that among the five deadenylases tested, Noc and Pan2 exhibited a biphasic expression which is out of phase to each other during adipocyte differentiation of 3T3-L1 cells. The expression levels of other deadenylases, including Parn, Ccr4, and Caf1, were relatively unchanged or reduced. The immediate early expressed Noc during 3T3-L1 adipogenesis was involved in regulating mitotic clonal expansion (MCE) and cyclin D1 expression, as demonstrated in Noc-silenced 3T3-L1 cells and Noc/primary mouse embryonic fibroblasts (MEFs). Transcriptional profiling of Noc-depleted 3T3-L1 adipocytes revealed that most of the differentially expressed genes were related to cell growth and proliferation. In human adipose tissue, NOC mRNA level negatively associated with both fasting serum insulin and homeostasis model assessment of insulin resistance, and positively associated with both adiponectin mRNA levels and circulating adiponectin levels. Taken together, these results suggest the role of Noc in the modulation of early adipogenesis as well as systemic insulin sensitivity. ? 2011 The Obesity Society. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84864357079&doi=10.1038%2foby.2012.37&partnerID=40&md5=f3c4c66124305a879549d5c974072423 https://scholars.lib.ntu.edu.tw/handle/123456789/495779 |
ISSN: | 1930-7381 | DOI: | 10.1038/oby.2012.37 | SDG/Keyword: | adenosine phosphate; adiponectin; cyclin D1; insulin; messenger RNA; nocturnin; protein Caf1; protein CCR4; protein Noc; protein PAN2; protein Parn; unclassified drug; adipocyte; adipogenesis; adult; animal cell; article; Caf1 gene; Ccr4 gene; cell differentiation; cell growth; cell proliferation; controlled study; embryo; enzyme regulation; female; fibroblast; gene; gene sequence; gene silencing; genetic transcription; homeostasis; human; human tissue; insulin blood level; insulin resistance; male; mouse; Noc gene; nonhuman; nucleotide sequence; Pan2 gene; Parn gene; protein depletion; protein expression; 3T3-L1 Cells; Adipogenesis; Adiponectin; Adipose Tissue; Adult; Animals; Cell Proliferation; Cyclin D1; Fasting; Fibroblasts; Gene Expression; Humans; Insulin; Insulin Resistance; Mice; Mice, Inbred C57BL; Mice, Knockout; Mitosis; Nuclear Proteins; Obesity; RNA, Messenger; Transcription Factors; Transcriptome |
Appears in Collections: | 醫學系 |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.