https://scholars.lib.ntu.edu.tw/handle/123456789/496158
Title: | Bioactivity-guided screening identifies pheophytin a as a potent anti-hepatitis C virus compound from Lonicera hypoglauca Miq. | Authors: | Wang S.-Y. Tseng C.-P. Tsai K.-C. Lin C.-F. Wen C.-Y. Tsay H.-S. Sakamoto N. CHIN-HSIAO TSENG Cheng J.-C. |
Issue Date: | 2009 | Journal Volume: | 385 | Journal Issue: | 2 | Start page/Pages: | 230-235 | Source: | Biochemical and Biophysical Research Communications | Abstract: | Chronic hepatitis C virus (HCV) infection is a worldwide public issue. In this study, we performed bioactivity-guided screening of the Lonicera hypoglauca Miq. crude extracts to find for naturally chemical entities with anti-HCV activity. Pheophytin a was identified from the ethanol-soluble fraction of L. hypoglauca that elicited dose-dependent inhibition of HCV viral proteins and RNA expression in both replicon cells and cell culture infectious system. Computational modeling revealed that pheophytin a can bind to the active site of HCV-NS3, suggesting that NS3 is a potent molecular target of pheophytin a. Biochemical analysis further revealed that pheophytin a inhibited NS3 serine protease activity with IC50 = 0.89 μM. Notably, pheophytin a and IFNα-2a elicited synergistic anti-HCV activity in replicon cells with no significant cytotoxicity. This study thereby demonstrates for the first time that pheophytin a is a potent HCV-NS3 protease inhibitor and offers insight for development of novel anti-HCV regimens. ? 2009 Elsevier Inc. All rights reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-67349091694&doi=10.1016%2fj.bbrc.2009.05.043&partnerID=40&md5=e8803187fcbf5ed48767725fe5dcbd38 https://scholars.lib.ntu.edu.tw/handle/123456789/496158 |
ISSN: | 0006-291X | DOI: | 10.1016/j.bbrc.2009.05.043 | SDG/Keyword: | alpha2a interferon; antivirus agent; nonstructural protein 3; pheophytin a; plant extract; serine proteinase; unclassified drug; virus protein; virus RNA; antiviral activity; article; cell culture; cell survival; cell viability; concentration response; controlled study; drug cytotoxicity; drug potency; drug potentiation; drug protein binding; drug screening; enzyme inhibition; gene expression; Hepatitis C virus; human; human cell; hydrogen bond; IC 50; Lonicera hypoglauca; medicinal plant; molecular model; nonhuman; plant leaf; plant stem; priority journal; protein expression; replicon; Antiviral Agents; Catalytic Domain; Cell Line; Computer Simulation; Drug Evaluation, Preclinical; Hepacivirus; Humans; Interferon Alfa-2a; Lonicera; Models, Molecular; Pheophytins; Viral Nonstructural Proteins; Viral Proteins; Virus Replication; Hepatitis C virus; Lonicera |
Appears in Collections: | 醫學系 |
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