https://scholars.lib.ntu.edu.tw/handle/123456789/500721
標題: | Rapid virtual hematoxylin and eosin histology of breast tissue specimens using a compact fluorescence nonlinear microscope | 作者: | CHI-KUANG SUN Cahill, L.C. Giacomelli, M.G. Yoshitake, T. Vardeh, H. Faulkner-Jones, B.E. Connolly, J.L. Sun, C.-K. Fujimoto, J.G. CHI-KUANG SUN |
公開日期: | 2018 | 卷: | 98 | 期: | 1 | 起(迄)頁: | 150-160 | 來源出版物: | Laboratory Investigation | 摘要: | Up to 40% of patients undergoing breast conserving surgery for breast cancer require repeat surgeries due to close to or positive margins. The lengthy processing required for evaluating surgical margins by standard paraffin-embedded histology precludes its use during surgery and therefore, technologies for rapid evaluation of surgical pathology could improve the treatment of breast cancer by reducing the number of surgeries required. We demonstrate real-time histological evaluation of breast cancer surgical specimens by staining specimens with acridine orange (AO) and sulforhodamine 101 (SR101) analogously to hematoxylin and eosin (H&E) and then imaging the specimens with fluorescence nonlinear microscopy (NLM) using a compact femtosecond fiber laser. A video-rate computational light absorption model was used to produce realistic virtual H&E images of tissue in real time and in three dimensions. NLM imaging could be performed to depths of 100 μm below the tissue surface, which is important since many surgical specimens require subsurface evaluation due to contamination artifacts on the tissue surface from electrocautery, surgical ink, or debris from specimen handling. We validate this method by expert review of NLM images compared to formalin-fixed, paraffin-embedded (FFPE) H&E histology. Diagnostically important features such as normal terminal ductal lobular units, fibrous and adipose stromal parenchyma, inflammation, invasive carcinoma, and in situ lobular and ductal carcinoma were present in NLM images associated with pathologies identified on standard FFPE H&E histology. We demonstrate that AO and SR101 were extracted to undetectable levels after FFPE processing and fluorescence in situ hybridization (FISH) HER2 amplification status was unaffected by the NLM imaging protocol. This method potentially enables cost-effective, real-time histological guidance of surgical resections. ? 2018 USCAP, Inc All rights reserved. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/500721 | DOI: | 10.1038/labinvest.2017.116 | SDG/關鍵字: | acridine orange; epidermal growth factor receptor 2; coloring agent; fluorescent dye; rhodamine; sulforhodamine 101; Article; artifact; autofluorescence; breast cancer; breast carcinoma; breast tissue; cauterization; collagen fiber; fluorescence; fluorescence in situ hybridization; fluorescence nonlinear microscopy; histopathology; human; human tissue; invasive carcinoma; light absorption; lobular carcinoma; microscopy; priority journal; specimen handling; breast; breast carcinoma in situ; breast tumor; chemistry; conservative treatment; cytology; female; fluorescence microscopy; immunology; intraoperative period; lobular carcinoma; mastectomy; nonlinear optical microscopy; Paget nipple disease; partial mastectomy; pathology; surgical margin; three dimensional imaging; tumor invasion; validation study; Acridine Orange; Breast; Breast Carcinoma In Situ; Breast Neoplasms; Carcinoma, Ductal, Breast; Carcinoma, Lobular; Coloring Agents; Female; Fluorescent Dyes; Humans; Imaging, Three-Dimensional; Intraoperative Period; Margins of Excision; Mastectomy; Mastectomy, Segmental; Microscopy, Fluorescence; Neoplasm Invasiveness; Nonlinear Optical Microscopy; Organ Sparing Treatments; Rhodamines |
顯示於: | 電機工程學系 |
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