https://scholars.lib.ntu.edu.tw/handle/123456789/502738
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Huang, Ru-Yin | en_US |
dc.contributor.author | CHIA-YIN LEE | en_US |
dc.creator | CHIA-YIN LEE;Huang, Ru-Yin | - |
dc.date.accessioned | 2020-06-16T09:02:20Z | - |
dc.date.available | 2020-06-16T09:02:20Z | - |
dc.date.issued | 2020-05 | - |
dc.identifier.issn | 0343-8651 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/502738 | - |
dc.description.abstract | Phosphatidylserine synthase (Pss) is involved in the metabolic pathway in phospholipid synthesis in different organisms. In this study, Pss expression in Vibrio parahaemolyticus was verified through liquid chromatography tandem-mass spectrometry. To analyze the characteristics of Pss, the recombinant Pss was overexpressed and purified from Escherichia coli. The optimum temperature and pH of Pss were 40 °C and 8, respectively. When reacting with divalent metal, Pss activity decreased. In addition, Pss could not only use cytidine diphosphate diacylglycerol (CDP-DAG, 16:0), but also CDP-DAG (18:1) as a substrate to produce cytidine 5'-monophosphate. Furthermore, the 3D structure of Pss was predicted, and the results revealed that histidine and lysine of the two HKD motifs were present in the catalytic site. Moreover, CDP-DAG (16:0) was docked with the Pss model. To investigate whether the two HKD motifs in Pss are important to its activity, site-directed mutagenesis of histidine was performed. The results revealed that the activities of both H131A and H352A were diminished. Little is known regarding the catalytic site of type I Pss. This is the first report on the biochemical characterization of Pss in V. parahaemolyticus. | en_US |
dc.language.iso | en | en_US |
dc.publisher | SPRINGER | en_US |
dc.relation.ispartof | Current microbiology | en_US |
dc.title | Characterization of a Phosphatidylserine Synthase of Vibrio parahaemolyticus | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1007/s00284-019-01854-x | - |
dc.identifier.pmid | 31897665 | - |
dc.identifier.scopus | 2-s2.0-85077286872 | - |
dc.identifier.isi | WOS:000505348300003 | - |
dc.identifier.url | https://api.elsevier.com/content/abstract/scopus_id/85077286872 | - |
dc.relation.pages | 710 | en_US |
dc.relation.journalvolume | 77 | en_US |
dc.relation.journalissue | 5 | en_US |
dc.relation.pageend | 715 | en_US |
item.fulltext | no fulltext | - |
item.openairetype | journal article | - |
item.languageiso639-1 | en | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
crisitem.author.dept | Agricultural Chemistry | - |
crisitem.author.orcid | 0000-0001-9917-2221 | - |
crisitem.author.parentorg | College of Bioresources and Agriculture | - |
顯示於: | 農業化學系 |
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