https://scholars.lib.ntu.edu.tw/handle/123456789/503998
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Liu C.-W. | en_US |
dc.contributor.author | Sung H.-C. | en_US |
dc.contributor.author | Lin S.-R. | en_US |
dc.contributor.author | Wu C.-W. | en_US |
dc.contributor.author | Lee C.-W. | en_US |
dc.contributor.author | Lee I.-T. | en_US |
dc.contributor.author | Yang Y.-F. | en_US |
dc.contributor.author | Yu I.-S. | en_US |
dc.contributor.author | SHU-WHA LIN | en_US |
dc.contributor.author | Chiang M.-H. | en_US |
dc.contributor.author | Liang C.-J. | en_US |
dc.contributor.author | YUH-LIEN CHEN | en_US |
dc.creator | Liu C.-W.;Sung H.-C.;Lin S.-R.;Wu C.-W.;Lee C.-W.;Lee I.-T.;Yang Y.-F.;Yu I.-S.;Shu-Wha Lin;Chiang M.-H.;Liang C.-J.;Chen Y.-L. | - |
dc.date.accessioned | 2020-06-22T07:36:59Z | - |
dc.date.available | 2020-06-22T07:36:59Z | - |
dc.date.issued | 2017 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/503998 | - |
dc.description.abstract | Resveratrol, an edible polyphenolic phytoalexin, improves endothelial dysfunction and attenuates inflammation. However, the mechanisms have not been thoroughly elucidated. Therefore, we investigated the molecular basis of the effects of resveratrol on TNF-α-induced ICAM-1 expression in HUVECs. The resveratrol treatment significantly attenuated the TNF-α-induced ICAM-1 expression. The inhibition of p38 phosphorylation mediated the reduction in ICAM-1 expression caused by resveratrol. Resveratrol also decreased TNF-α-induced IκB phosphorylation and the phosphorylation, acetylation, and translocation of NF-κB p65. Moreover, resveratrol induced the AMPK phosphorylation and the SIRT1 expression in TNF-α-treated HUVECs. Furthermore, TNF-α significantly suppressed miR-221/-222 expression, which was reversed by resveratrol. miR-221/-222 overexpression decreased p38/NF-κB and ICAM-1 expression, which resulted in reduced monocyte adhesion to TNF-α-treated ECs. In a mouse model of acute TNF-α-induced inflammation, resveratrol effectively attenuated ICAM-1 expression in the aortic ECs of TNF-α-treated wild-type mice. These beneficial effects of resveratrol were lost in miR-221/222 knockout mice. Our data showed that resveratrol counteracted the TNF-α-mediated reduction in miR-221/222 expression and decreased the TNF-α-induced activation of p38 MAPK and NF-κB, thereby suppressing ICAM-1 expression and monocyte adhesion. Collectively, our results show that resveratrol attenuates endothelial inflammation by reducing ICAM-1 expression and that the protective effect was mediated partly through the miR-221/222/AMPK/p38/NF-κB pathway. ? 2017 The Author(s). | - |
dc.relation.ispartof | Scientific Reports | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | antiinflammatory agent; hydroxymethylglutaryl coenzyme A reductase kinase; intercellular adhesion molecule 1; microRNA; MIRN221 microRNA, human; MIRN222 microRNA, human; mitogen activated protein kinase p38; resveratrol; SIRT1 protein, human; sirtuin 1; stilbene derivative; transcription factor RelA; tumor necrosis factor; animal; antagonists and inhibitors; C57BL mouse; cell adhesion; chemically induced; cytology; disease model; drug effect; gene expression regulation; genetics; human; inflammation; knockout mouse; metabolism; monocyte; mouse; pathology; peritonitis; primary cell culture; signal transduction; umbilical vein endothelial cell; AMP-Activated Protein Kinases; Animals; Anti-Inflammatory Agents; Cell Adhesion; Disease Models, Animal; Gene Expression Regulation; Human Umbilical Vein Endothelial Cells; Humans; Inflammation; Intercellular Adhesion Molecule-1; Mice; Mice, Inbred C57BL; Mice, Knockout; MicroRNAs; Monocytes; p38 Mitogen-Activated Protein Kinases; Peritonitis; Primary Cell Culture; Signal Transduction; Sirtuin 1; Stilbenes; Transcription Factor RelA; Tumor Necrosis Factor-alpha | - |
dc.title | Resveratrol attenuates ICAM-1 expression and monocyte adhesiveness to TNF-α-treated endothelial cells: Evidence for an anti-inflammatory cascade mediated by the MIR-221/222/AMPK/p38/NF-7kappa;B pathway | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1038/srep44689 | - |
dc.identifier.pmid | 28338009 | - |
dc.identifier.scopus | 2-s2.0-85016160024 | - |
dc.relation.pages | 44689 | - |
dc.relation.journalvolume | 7 | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
crisitem.author.dept | Clinical Laboratory Sciences and Medical Biotechnology | - |
crisitem.author.dept | Anatomy and Cell Biology | - |
crisitem.author.orcid | 0000-0001-6748-5581 | - |
crisitem.author.orcid | 0000-0002-6498-4008 | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
顯示於: | 醫學檢驗暨生物技術學系 |
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