https://scholars.lib.ntu.edu.tw/handle/123456789/507122
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Chang Y.-Y. | en_US |
dc.contributor.author | HUI-MIN SU | en_US |
dc.contributor.author | Chen S.-H. | en_US |
dc.contributor.author | Hsieh W.-T. | en_US |
dc.contributor.author | Chyuan J.-H. | en_US |
dc.contributor.author | Chao P.-M. | en_US |
dc.date.accessioned | 2020-06-30T07:36:31Z | - |
dc.date.available | 2020-06-30T07:36:31Z | - |
dc.date.issued | 2016 | - |
dc.identifier.issn | 2072-6643 | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85006757525&doi=10.3390%2fnu8120805&partnerID=40&md5=d98641748840bc526d32a6bb4b24cfad | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/507122 | - |
dc.description.abstract | We previously reported that bitter melon seed oil (BMSO) was an effective anti-steatosis and antiobesity agent. Since the major fatty acid α-eleostearic acid (α-ESA) in BMSO is a peroxisome proliferator-activated receptor α (PPARα) activator, the objective was to investigate the role of PPARα in BMSO-modulated lipid disorders and α-ESA metabolism. C57BL/6J wild (WD) and PPARα knockout (KO) mice were fed a high-fat diet containing BMSO (15% soybean oil + 15% BMSO, HB) or not (30% soybean oil, HS) for 5 weeks. The HB diet significantly reduced hepatic triglyceride concentrations and increased acyl-CoA oxidase activity in WD, but not in KO mice. However, regardless of genotype, body fat percentage was lowered along with upregulated protein levels of uncoupling protein 1 (UCP1) and tyrosine hydroxylase, as well as signaling pathway of cAMP-dependent protein kinase and AMP-activated protein kinase in the white adipose tissue of HB-treated groups compared to HS cohorts. In WD-HB and KO-HB groups, white adipose tissue had autophagy, apoptosis, inflammation, and browning characteristics. Without PPARα, in vivo reduction of α-ESA into rumenic acid was slightly but significantly lowered, along with remarkable reduction of hepatic retinol saturase (RetSat) expression. We concluded that BMSO-mediated anti-steatosis depended on PPARα, whereas the anti-adiposity effect was PPARα-independent. In addition, PPARα-dependent enzymes may participate in α-ESA conversion, but only have a minor role. ? 2016 by the authors; licensee MDPI, Basel, Switzerland. | - |
dc.publisher | MDPI AG | - |
dc.relation.ispartof | Nutrients | - |
dc.subject | Bitter melon seed oil; Hepatic steatosis; Obesity; PPARα; α-eleostearic acid | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | acyl coenzyme A oxidase; alpha eleostearic acid; bitter melon seed oil; cyclic AMP dependent protein kinase; hydroxymethylglutaryl coenzyme A reductase kinase; messenger RNA; peroxisome proliferator activated receptor alpha; polyunsaturated fatty acid; retinol; rumenic acid; soybean oil; stearic acid; triacylglycerol; tyrosine 3 monooxygenase; unclassified drug; uncoupling protein 1; vegetable oil; 9,11-linoleic acid; acyl coenzyme A oxidase; conjugated linoleic acid; cyclic AMP dependent protein kinase; eleostearic acid; linolenic acid; oxidoreductase; peroxisome proliferator activated receptor alpha; retinol saturase (all trans retinol 13,14 reductase), mouse; triacylglycerol; tyrosine 3 monooxygenase; Ucp1 protein, mouse; uncoupling protein 1; vegetable oil; animal experiment; animal model; animal tissue; apoptosis; Article; autophagy; biochemical analysis; body weight; cell death; controlled study; dietary intake; disorders of lipid and lipoprotein metabolism; enzyme activity; fat content; gas chromatography; gene expression; histology; lipid analysis; male; Momordica charantia; mouse; nonhuman; obesity; real time polymerase chain reaction; RNA isolation; Western blotting; adverse effects; animal; C57BL mouse; chemistry; drug effects; Dyslipidemias; fatty liver; genetics; knockout mouse; lipid diet; liver; metabolism; Momordica charantia; physiology; phytotherapy; signal transduction; white adipose tissue; Acyl-CoA Oxidase; Adipose Tissue, White; Adiposity; Animals; Cyclic AMP-Dependent Protein Kinases; Diet, High-Fat; Dyslipidemias; Fatty Liver; Linoleic Acids, Conjugated; Linolenic Acids; Liver; Mice; Mice, Inbred C57BL; Mice, Knockout; Momordica charantia; Oxidoreductases Acting on CH-CH Group Donors; Phytotherapy; Plant Oils; PPAR alpha; Signal Transduction; Triglycerides; Tyrosine 3-Monooxygenase; Uncoupling Protein 1 | - |
dc.title | Roles of peroxisome proliferator-activated receptor α in bitter melon seed oil-corrected lipid disorders and conversion of α-eleostearic acid into rumenic acid in C57Bl/6J mice | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.3390/nu8120805 | - |
dc.identifier.pmid | 27973445 | - |
dc.identifier.scopus | 2-s2.0-85006757525 | - |
dc.relation.pages | 805 | - |
dc.relation.journalvolume | 8 | - |
dc.relation.journalissue | 12 | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
crisitem.author.dept | Physiology | - |
crisitem.author.orcid | 0000-0002-9970-4459 | - |
crisitem.author.parentorg | College of Medicine | - |
顯示於: | 生理學科所 |
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