https://scholars.lib.ntu.edu.tw/handle/123456789/507131
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Chou Y.-C. | en_US |
dc.contributor.author | HUI-MIN SU | en_US |
dc.contributor.author | Lai T.-W. | en_US |
dc.contributor.author | Chyuan J.-H. | en_US |
dc.contributor.author | Chao P.-M. | en_US |
dc.creator | Chou Y.-C.;Hui-Min Su;Lai T.-W.;Chyuan J.-H.;Chao P.-M. | - |
dc.date.accessioned | 2020-06-30T07:36:33Z | - |
dc.date.available | 2020-06-30T07:36:33Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 0899-9007 | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84862180021&doi=10.1016%2fj.nut.2011.11.019&partnerID=40&md5=cb4ac98c21ad69bf6791f91590937f25 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/507131 | - |
dc.description.abstract | Objective: The aim of this study was to investigate the adipogenic effect of cis-9, trans-11, trans-13-conjugated linolenic acid (c9, t11, t13-CLN), a fatty acid naturally present in bitter melon. Methods: The 3T3-L1 murine preadipocyte cell line was used to test the effect of saponifiables from whole bitter melon and of commercially prepared pure c9, t11, t13-CLN on adipocyte differentiation. The effect of c9, t11, t13. -CLN on 3T3-L1 cell viability was also tested at proliferation, mitotic clonal expansion, and terminal differentiation stages. Results: Compared to the free fatty acid control mixture, the proadipogenic effect on 3T3-L1 was less potent using saponifiables obtained from bitter melon. C9, t11, t13-CLN, unlike its non-conjugated counterpart linolenic acid (LN) or other common fatty acids such as oleic acid or linoleic acid, exerted no proadipogenic effect on 3T3-L1. In contrast to LN displaying no cytotoxic effect at a concentration ?100 μM, c9, t11, t13-CLN caused a dose-dependent reduction in the viability of pre- and postconfluent preadipocytes associated with apoptosis. Sustained ERK/MAPK activation, accompanied by increased peroxisome proliferator-activated receptor γ phosphorylation, was seen in c9, t11, t13-CLN-treated cells at initiation of differentiation. Conclusion: C9, t11, t13-CLN is less adipogenic for 3T3-L1 cells than LN and this is partly due to its apoptotic effect on proliferating preadipocytes and to the sustained ERK phosphorylation seen during mitotic clonal expansion. ? 2012 Elsevier Inc. | - |
dc.relation.ispartof | Nutrition | - |
dc.subject | 3T3-L1; Adipogenesis; Cis-9; ERK/MAPK; Linolenic acid; Trans-11; Trans-13 onjugated linolenic acid | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | cis 9, trans 11, trans 13 conjugated linolenic acid; fatty acid; linolenic acid; mitogen activated protein kinase; peroxisome proliferator activated receptor gamma; unclassified drug; adipocyte; adipogenesis; animal cell; apoptosis; article; cell clone; cell differentiation; cell expansion; cell proliferation; cell strain 3T3; cell viability; concentration response; controlled study; cytotoxicity; enzyme activation; enzyme phosphorylation; Momordica charantia; nonhuman; priority journal; proadipocyte; protein phosphorylation; 3T3-L1 Cells; Adipocytes; Adipogenesis; Animals; Anti-Obesity Agents; Apoptosis; Cell Proliferation; Cell Survival; Extracellular Signal-Regulated MAP Kinases; Fruit; Gene Expression Regulation; Linolenic Acids; Mice; Momordica charantia; Osmolar Concentration; Phosphorylation; PPAR gamma; Protein Processing, Post-Translational; RNA, Messenger; Triglycerides; Cucumis; Murinae | - |
dc.title | Cis-9, trans-11, trans-13-conjugated linolenic acid induces apoptosis and sustained ERK phosphorylation in 3T3-L1 preadipocytes | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1016/j.nut.2011.11.019 | - |
dc.identifier.pmid | 22465903 | - |
dc.identifier.scopus | 2-s2.0-84862180021 | - |
dc.relation.pages | 803-811 | - |
dc.relation.journalvolume | 28 | - |
dc.relation.journalissue | 7/8 | - |
item.fulltext | no fulltext | - |
item.grantfulltext | none | - |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.cerifentitytype | Publications | - |
crisitem.author.dept | Physiology | - |
crisitem.author.orcid | 0000-0002-9970-4459 | - |
crisitem.author.parentorg | College of Medicine | - |
顯示於: | 生理學科所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。