https://scholars.lib.ntu.edu.tw/handle/123456789/507626
標題: | Increased serum interleukin-17 and peripheral Th17 cells in children with acute Henoch-Schönlein purpura | 作者: | Jen H.-Y. YA-HUI CHUANG Lin S.-C. BOR-LUEN CHIANG YAO-HSU YANG |
公開日期: | 2011 | 卷: | 22 | 期: | 8 | 起(迄)頁: | 862-868 | 來源出版物: | Pediatric Allergy and Immunology | 摘要: | Background: Interleukin (IL)-17 and Th17 cells have been involved in many autoimmune diseases. The aim of this study is to investigate the involvement of IL-17 and Th17 cells in the pathogenesis of childhood Henoch-Sch?nlein purpura (HSP). Methods: Serum and supernatant levels of cytokines and chemokines were analyzed by enzyme-linked immunosorbent assay (ELISA). Using intracellular staining, the frequency of peripheral Th17 and Th1 cells was studied by flow cytometry. Results: Children with acute HSP had significantly higher serum levels of IL-17, IL-6 and transforming growth factor-β than healthy controls. The IL-17 levels in culture supernatants of peripheral blood mononuclear cells with anti-CD3 and CD28 antibody stimulation were much higher in patients with HSP (281.2±91.4 vs. 47.7±22.6pg/ml, p=0.022). The patients also had more Th17 cells (1.67±0.36% vs. 0.71±0.15%, p=0.033) but not Th1 cells in peripheral blood. Moreover, IL-17 could promote human endothelial cells to produce chemoattractants IL-8 and monocyte chemotactic protein-1. Conclusion: The increased frequency of peripheral Th17 cells and serum IL-17 levels are shown in childhood HSP that may in part contribute to vascular inflammation, suggesting cellular immunity is likely to be involved in the process of HSP. ? 2011 John Wiley & Sons A/S. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/507626 | ISSN: | 0905-6157 | DOI: | 10.1111/j.1399-3038.2011.01198.x | SDG/關鍵字: | CD28 antigen; CD3 antigen; interleukin 17; interleukin 23; interleukin 6; interleukin 8; monocyte chemotactic protein 1; transforming growth factor beta; anaphylactoid purpura; article; blood level; cellular immunity; child; clinical article; controlled study; cytokine production; endothelium cell; enzyme linked immunosorbent assay; female; flow cytometry; human; human cell; inflammation; male; pathogenesis; peripheral blood mononuclear cell; priority journal; supernatant; Th1 cell; Th17 cell; Acute Disease; Adolescent; Cell Separation; Cells, Cultured; Chemokine CCL2; Child; Child, Preschool; Endothelial Cells; Female; Flow Cytometry; Gene Expression Regulation; Humans; Immunization; Interleukin-17; Interleukin-6; Male; Purpura, Schoenlein-Henoch; Th1 Cells; Th17 Cells; Transforming Growth Factor beta |
顯示於: | 醫學檢驗暨生物技術學系 |
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