https://scholars.lib.ntu.edu.tw/handle/123456789/508075
標題: | Quantitative competitive reverse transcription-PCR for quantification of dengue virus RNA | 作者: | Wang W.-K. Lee C.-N. CHUAN-LIANG KAO Lin Y.-L. King C.-C. |
公開日期: | 2000 | 卷: | 38 | 期: | 9 | 起(迄)頁: | 3306-3310 | 來源出版物: | Journal of Clinical Microbiology | 摘要: | A quantitative competitive reverse transcription-PCR assay was developed to quantify dengue virus RNA in this study. The main features include a primer pair targeting a highly conserved region in the capsid and the addition of competing RNA that contains an internal deletion to provide a stringent internal control for quantification. It can be utilized to quantify RNA isolated from the four dengue virus serotypes but not RNA isolated from other flaviviruses, including Japanese encephalitis virus and hepatitis C virus, both prevalent in Asia. It can also be used to quantify dengue virus RNA isolated from the plasma of infected individuals. The sensitivity of the assay was estimated to be 10 to 50 copies of RNA per reaction, and twofold differences in virus titer are distinguishable. This assay is a convenient, sensitive, and accurate method for quantification and can be used to further understanding of the pathogenesis of dengue virus infection. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/508075 | ISSN: | 0095-1137 | DOI: | 10.1128/jcm.38.9.3306-3310.2000 | SDG/關鍵字: | virus RNA; article; controlled study; dengue; Dengue virus; gene deletion; gene isolation; Hepatitis C virus; Japanese encephalitis virus; nonhuman; priority journal; quality control; reverse transcription polymerase chain reaction; serotype; species difference; virus capsid; virus titration; Dengue virus; Hepatitis C virus; Japanese encephalitis virus; Miridae; RNA viruses |
顯示於: | 醫學檢驗暨生物技術學系 |
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