https://scholars.lib.ntu.edu.tw/handle/123456789/508235
標題: | Rapid identification of Mycobacterium tuberculosis infection by a new array format-based surface plasmon resonance method | 作者: | Hsieh S.-C. Chang C.-C. Lu C.-C. Wei C.-F. Lin C.-S. HSIN-CHIH LAI Lin C.-W. |
公開日期: | 2012 | 卷: | 7 | 起(迄)頁: | 1-6 | 來源出版物: | Nanoscale Research Letters | 摘要: | Tubercle bacillus [TB] is one of the most important chronic infectious diseases that cause millions of deaths annually. While conventional smear microscopy and culture methods are widely used for diagnosis of TB, the former is insensitive, and the latter takes up to 6 to 8 weeks to provide a result, limiting the value of these methods in aiding diagnosis and intermediate decisions on treatment. Therefore, a rapid detection method is essential for the diagnosis, prognosis assessment, and recurrence monitoring. A new surface plasmon resonance [SPR] biosensor based on an array format, which allowed immobilizing nine TB antigens onto the sensor chip, was constructed. Simultaneous determination of multiple TB antibodies in serum had been accomplished with this array-based SPR system. The results were compared with enzyme-linked immunosorbent assay, a conventional immunological method. Array-based SPR showed more advantages in providing label-free and real-time detection. Additionally, the high sensitivity and specificity for the detection of TB infection showed its potential for future development of biosensor arrays for TB diagnosis. ? 2012 Hsieh et al. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/508235 | ISSN: | 1931-7573 | DOI: | 10.1186/1556-276X-7-180 | SDG/關鍵字: | Antigens; Bacteriology; Biosensors; Diagnosis; Identification (control systems); Plasmons; Terbium; Biosensor arrays; Chronic infectious disease; Enzyme linked immunosorbent assay; Immunological methods; Label-free and real-time detections; Mycobacterium tuberculosis; Rapid identification; Simultaneous determinations; Surface plasmon resonance; Mycobacterium tuberculosis |
顯示於: | 醫學檢驗暨生物技術學系 |
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