https://scholars.lib.ntu.edu.tw/handle/123456789/508560
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Sheu M.L. | en_US |
dc.contributor.author | CHIH-KANG CHIANG | en_US |
dc.contributor.author | KEH-SUNG TSAI | en_US |
dc.contributor.author | Ho F.M. | en_US |
dc.contributor.author | TE-I WENG | en_US |
dc.contributor.author | Wu H.Y. | en_US |
dc.contributor.author | Liu S.H. | en_US |
dc.creator | Sheu M.L.;Chiang C.K.;Tsai K.S.;Ho F.M.;Te-I Weng;Wu H.Y.;Liu S.H. | - |
dc.date.accessioned | 2020-07-05T01:54:05Z | - |
dc.date.available | 2020-07-05T01:54:05Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 0891-5849 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/508560 | - |
dc.description.abstract | Angiopathy is a major complication of diabetes. Abnormally high blood glucose is a crucial risk factor for endothelial cell damage. Nuclear factor-κB (NF-κB) has been demonstrated as a mediated signaling in hyperglycemia or oxidative stress-triggered apoptosis of endothelial cells. Here we explored the efficacy of honokiol, a small molecular weight natural product, on NADPH oxidase-related oxidative stress-mediated NF-κB-regulated signaling and apoptosis in human umbilical vein endothelial cells (HUVECs) under hyperglycemic conditions. The methods of morphological Hoechst staining and annexin V/propidium iodide staining were used to detect apoptosis. Submicromolar concentrations of honokiol suppressed the increases of NADPH oxidase activity, Rac-1 phosphorylation, p22phox protein expression, and reactive oxygen species production in high glucose (HG)-stimulated HUVECs. The degradation of IκBα and increase of NF-κB activity were inhibited by honokiol in HG-treated HUVECs. Moreover, honokiol (0.125-1?μM) also suppressed HG-induced cyclooxygenase (COX)-2 upregulation and prostaglandin E2 production in HUVECs. Honokiol could reduce increased caspase-3 activity and the subsequent apoptosis and cell death triggered by HG. These results imply that inhibition of NADPH oxidase-related oxidative stress by honokiol suppresses the HG-induced NF-κB-regulated COX-2 upregulation, apoptosis, and cell death in HUVECs, which has the potential to be developed as a therapeutic agent to prevent hyperglycemia-induced endothelial damage. ? 2008 Elsevier B.V. All rights reserved. | - |
dc.relation.ispartof | Free Radical Biology and Medicine | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | glucose; honokiol; protein p22; Rac1 protein; reduced nicotinamide adenine dinucleotide phosphate oxidase; apoptosis; article; controlled study; endothelium cell; enzyme activity; human; human cell; oxidative stress; priority journal; protein expression; protein phosphorylation; Apoptosis; Biphenyl Compounds; Cyclooxygenase 2; Endothelial Cells; Endothelium, Vascular; Enzyme Activation; Glucose; Humans; Lignans; NADPH Oxidase; NF-kappa B; Oxidative Stress; Phosphorylation; rac1 GTP-Binding Protein; Reactive Oxygen Species; Signal Transduction; Umbilical Veins | - |
dc.title | Inhibition of NADPH oxidase-related oxidative stress-triggered signaling by honokiol suppresses high glucose-induced human endothelial cell apoptosis | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1016/j.freeradbiomed.2008.03.014 | - |
dc.identifier.pmid | 18423412 | - |
dc.identifier.scopus | 2-s2.0-43849110615 | - |
dc.relation.pages | 2043-2050 | - |
dc.relation.journalvolume | 44 | - |
dc.relation.journalissue | 12 | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
crisitem.author.dept | Toxicology | - |
crisitem.author.dept | Integrated Diagnostics and Therapeutics-NTUH | - |
crisitem.author.dept | Internal Medicine | - |
crisitem.author.dept | Internal Medicine-NTUH | - |
crisitem.author.dept | Institute of Food Safety and Health | - |
crisitem.author.dept | Center for Biotechnology | - |
crisitem.author.dept | Laboratory Medicine | - |
crisitem.author.dept | Laboratory Medicine-NTUH | - |
crisitem.author.dept | Forensic Medicine | - |
crisitem.author.dept | Emergency Medicine-NTUH | - |
crisitem.author.orcid | 0000-0001-9021-4616 | - |
crisitem.author.orcid | 0000-0001-8528-1566 | - |
crisitem.author.orcid | 0000-0002-8797-1672 | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Public Health | - |
crisitem.author.parentorg | Others: University-Level Research Centers | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
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