https://scholars.lib.ntu.edu.tw/handle/123456789/511449
Title: | Interference caused by the contents of serum separator tubes in the Vitros CRP assay | Authors: | Chang C.-Y. JIN-YING LU Chien T.-I. JAU-TSUEN KAO MEI-CHUN LIN Shih P.-C. Yan S.-N. |
Issue Date: | 2003 | Journal Volume: | 40 | Journal Issue: | 3 | Start page/Pages: | 249-251 | Source: | Annals of Clinical Biochemistry | Abstract: | Background: We have observed discrepancies between C-reactive protein (CRP) measured in serum prepared from a serum separator tube (SST) and that obtained from a plain tube, when using the Vitros CRP assay. Our study aimed at elucidating the cause of these discrepancies. Methods: Eighty-seven specimens from hospitalized patients with various types of inflammatory disease were analysed using a fixed-point immuno-rate method on a Vitros CRP slide. The serum was prepared simultaneously in both vacuum and SSTs. We also performed mixing tests by adding 47 samples of serum prepared from plain tubes to SSTs and incubating for 15 min before CRP analysis. Results: Lower values of CRP were found in serum prepared from plain tubes than in serum from SSTs. Addition of serum prepared from plain tubes to SSTs and incubating for 15 min increased the CRP values significantly. The ratio of CRP measured in serum prepared from plain tubes and from SSTs did not differ significantly from the ratio obtained when serum was prepared in a plain tube then added to an SST. Discussion: We propose that SSTs can adsorb some macromolocules that form complexes with CRP. The addition of SST gel to serum results in the release of CRP molecules from these complexes, which enhances the antigen-antibody reaction on the Vitros CRP slide and increases the measured CRP concentrations. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0038704785&doi=10.1258%2f000456303321610556&partnerID=40&md5=7e78ca2143f38529d7b732663584d201 https://scholars.lib.ntu.edu.tw/handle/123456789/511449 |
ISSN: | 0004-5632 | DOI: | 10.1258/000456303321610556 | SDG/Keyword: | C reactive protein; analytic method; analytical error; antigen antibody reaction; article; blood sampling; comparative study; complex formation; controlled study; device; hospital patient; human; incubation time; inflammatory disease; macromolecule; priority journal; protein blood level; protein determination; quantitative analysis; separation technique; serum; tube; vacuum; Antibodies, Monoclonal; Blood Chemical Analysis; C-Reactive Protein; False Negative Reactions; Horseradish Peroxidase; Humans; Reagent Kits, Diagnostic; Regression Analysis |
Appears in Collections: | 醫學系 |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.