https://scholars.lib.ntu.edu.tw/handle/123456789/513347
標題: | Organic anion-transporting polypeptide 1B3 as a dual reporter gene for fluorescence and magnetic resonance imaging | 作者: | Wu M.-R. HON-MAN LIU Lu C.-W. Shen W.-H. Lin I.-J. Liao L.-W. YI-YOU HUANG MING-JIUM SHIEH Hsiao J.-K. |
公開日期: | 2018 | 卷: | 32 | 期: | 3 | 起(迄)頁: | 1705-1715 | 來源出版物: | FASEB Journal | 摘要: | Reporter proteins have broad applications in visualizing molecular events at the cellular, tissue and whole-body levels. Transmembrane transporters recognizing specificmolecular domains are of particular interest because they enable the migration of signal-source molecules from the extracellular space to the cytoplasm for subsequent application in multimodality imaging. Organic anion-transporting polypeptides (OATPs) have demonstrated their MRI reporter efficacy. We further expanded their use as a dual-modality reporter in MRI and noninvasive in vivo imaging system (IVIS). We overexpressed OATP1B3 in the HT-1080 sarcoma cell line. Both Gd-EOB-DTPA, anMRI contrast agent, and indocyanine green (ICG), a near-infrared fluorescent dye that provides better deep-tissue detection because of its longwavelength, couldbe deliveredto the intracellular space and imaged in a tumor-bearing nudemousemodel. Our in vivo dual-imaging reporter systemachieved high sensitivity inMRI and observation periods lasting as long as 96 h in IVIS. Because of the superior temporal and spatial resolutions and the clinical availability of both ICG and Gd-EOB-DTPA, this dual-imaging OATP1B3 systemwill find biomedical use in tumor biology, stemcell trafficking, and tissue engineering. ? The Author(s). |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/513347 | ISSN: | 0892-6638 | DOI: | 10.1096/fj.201700767R | SDG/關鍵字: | gadoxetic acid; indocyanine green; solute carrier organic anion transporter 1B3; SLCO1B1 protein, human; solute carrier organic anion transporter 1B1; animal cell; Article; cell migration; controlled study; female; fluorescence; HT-1080 cell line; in vivo study; intracellular space; mouse; nonhuman; nuclear magnetic resonance imaging; priority journal; protein expression; reporter gene; stem cell; tissue engineering; animal; Bagg albino mouse; cancer transplantation; diagnostic imaging; fluorescence imaging; genetics; human; metabolism; nude mouse; sarcoma; tumor cell line; xenograft; Animals; Cell Line, Tumor; Genes, Reporter; Heterografts; Humans; Magnetic Resonance Imaging; Mice; Mice, Inbred BALB C; Mice, Nude; Neoplasm Transplantation; Optical Imaging; Sarcoma; Solute Carrier Organic Anion Transporter Family Member 1b1 |
顯示於: | 醫學院附設醫院 (臺大醫院) |
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