https://scholars.lib.ntu.edu.tw/handle/123456789/513427
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Hsiao J.-K. | en_US |
dc.contributor.author | Tai M.-F. | en_US |
dc.contributor.author | Chu H.-H. | en_US |
dc.contributor.author | Chen S.-T. | en_US |
dc.contributor.author | Li H. | en_US |
dc.contributor.author | DAR-MING LAI | en_US |
dc.contributor.author | SUNG-TSANG HSIEH | en_US |
dc.contributor.author | Wang J.-L. | en_US |
dc.contributor.author | HON-MAN LIU | en_US |
dc.creator | Hsiao J.-K.;Tai M.-F.;Chu H.-H.;Chen S.-T.;Li H.;Lai D.-M.;Hsieh S.-T.;Wang J.-L.;Hon-Man Liu | - |
dc.date.accessioned | 2020-08-21T08:51:09Z | - |
dc.date.available | 2020-08-21T08:51:09Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 0740-3194 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/513427 | - |
dc.description.abstract | The purpose of this work was to evaluate the efficacy of labeling human mesenchymal stem cells (hMSCs) by ionic superparamagnetic iron oxide (SPIO) without a transfection agent and verifying its capability to be detected with clinical 1.5 T magnetic resonance (MR) at the single-cell level. Human hMSCs were incubated for 24 h with an ionic SPIO, Ferucarbotran. The labeling efficiency of hMSCs was determined by iron content measurement spectrophotometrically, and the influence of labeling on cell behavior was ascertained by examination of cell viability using the trypan blue exclusion method, cell proliferation analysis using MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide) assay, mitochondrial membrane potential (MMP) change, differentiation capacity, and reactive oxygen species (ROS) production measured by dichlorofluorescein diacetate (DCFDA) fluorescent probe. Labeled hMSCs were scanned under 1.5 T MRI with three-dimensional (3D) and two-dimensional (2D) T2-weighted gradient echo (GRE) pulse sequences. Human hMSC labeling without transfection agent was efficient. The iron content in hMSCs was 23.4 pg Fe/cell. No significant change was found in viability, proliferation, MMP change, ROS production, or differentiation capacity. About 45.2% of the hMSCs could be detected using 1.5 T MRI at the single cell level with 3D GRE and four repetitions. © 2007 Wiley-Liss, Inc. | en_US |
dc.language.iso | en | en_US |
dc.relation.ispartof | Magnetic Resonance in Medicine | en_US |
dc.title | Magnetic nanoparticle labeling of mesenchymal stem cells without transfection agent: Cellular behavior and capability of detection with clinical 1.5 T magnetic resonance at the single cell level | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1002/mrm.21377 | - |
dc.identifier.pmid | 17899592 | - |
dc.identifier.scopus | 2-s2.0-35048831742 | - |
dc.relation.pages | 717-724 | en_US |
dc.relation.journalvolume | 58 | en_US |
dc.relation.journalissue | 4 | en_US |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | none | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
crisitem.author.dept | Surgery-NTUH | - |
crisitem.author.dept | Surgery | - |
crisitem.author.dept | Anatomy and Cell Biology | - |
crisitem.author.dept | Neurology-NTUH | - |
crisitem.author.dept | Neurology | - |
crisitem.author.dept | Radiology | - |
crisitem.author.dept | Medical Imaging-NTUH | - |
crisitem.author.orcid | 0000-0002-9896-5251 | - |
crisitem.author.orcid | 0000-0002-3188-8482 | - |
crisitem.author.orcid | 0000-0001-6715-6366 | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
顯示於: | 醫學院附設醫院 (臺大醫院) |
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