https://scholars.lib.ntu.edu.tw/handle/123456789/516655
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | A-H Liao | en_US |
dc.contributor.author | H-L Liu | en_US |
dc.contributor.author | C-H Su | en_US |
dc.contributor.author | M-Y Hua | en_US |
dc.contributor.author | H-W Yang | en_US |
dc.contributor.author | Y-T Weng | en_US |
dc.contributor.author | P-H Hsu | en_US |
dc.contributor.author | S-M Huang | en_US |
dc.contributor.author | S-Y Wu | en_US |
dc.contributor.author | H-El Wang | en_US |
dc.contributor.author | T-C Yen | en_US |
dc.contributor.author | P-C Li | en_US |
dc.contributor.author | HAO-LI LIU | en_US |
dc.contributor.author | A-H Liao | en_US |
dc.contributor.author | H-L Liu | en_US |
dc.contributor.author | C-H Su | en_US |
dc.contributor.author | M-Y Hua | en_US |
dc.contributor.author | H-W Yang | en_US |
dc.contributor.author | Y-T Weng | en_US |
dc.contributor.author | P-H Hsu | en_US |
dc.contributor.author | S-M Huang | en_US |
dc.contributor.author | S-Y Wu | en_US |
dc.contributor.author | H-El Wang | en_US |
dc.contributor.author | T-C Yen | en_US |
dc.contributor.author | P-C Li | en_US |
dc.contributor.author | PAI-CHI LI | en_US |
dc.creator | A-H Liao;H-L Liu;C-H Su;M-Y Hua;H-W Yang;Y-T Weng;P-H Hsu;S-M Huang;S-Y Wu;H-El Wang;T-C Yen;P-C Li | - |
dc.date.accessioned | 2020-10-07T01:24:54Z | - |
dc.date.available | 2020-10-07T01:24:54Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 319155 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/516655 | - |
dc.description.abstract | This paper presents new albumin-shelled Gd-DTPA microbubbles (MBs) that can concurrently serve as a dual-modality contrast agent for ultrasound (US) imaging and magnetic resonance (MR) imaging to assist bloodbrain barrier (BBB) opening and detect intracerebral hemorrhage (ICH) during focused ultrasound brain drug delivery. Perfluorocarbon-filled albumin-(Gd-DTPA) MBs were prepared with a mean diameter of 2320 nm and concentration of 2.903×10 9MBs ml -1using albumin-(Gd-DTPA) and by sonication with perfluorocarbon (C 3F 8) gas. The albumin-(Gd-DTPA) MBs were then centrifuged and the procedure was repeated until the free Gd 3+ions were eliminated (which were detected by the xylenol orange sodium salt solution). The albumin-(Gd-DTPA) MBs were also characterized and evaluated both in vitro and in vivo by US and MR imaging. Focused US was used with the albumin-(Gd-DTPA) MBs to induce disruption of the BBB in 18 rats. BBB disruption was confirmed with contrast-enhanced T 1-weighted turbo-spin-echo sequence MR imaging. Heavy T 2*-weighted 3D fast low-angle shot sequence MR imaging was used to detect ICH. In vitro US imaging experiments showed that albumin-(Gd-DTPA) MBs can significantly enhance the US contrast in T 1-, T 2- and T 2*-weighted MR images. The r 1and r 2relaxivities for Gd-DTPA were 7.69 and 21.35 s -1mM -1, respectively, indicating that the MBs represent a positive contrast agent in T 1-weighted images. In vivo MR imaging experiments on 18 rats showed that focused US combined with albumin-(Gd-DTPA) MBs can be used to both induce disruption of the BBB and detect ICH. To compare the signal intensity change between pure BBB opening and BBB opening accompanying ICH, albumin-(Gd-DTPA) MB imaging can provide a ratio of 5.14 with significant difference (p = 0.026), whereas Gd-DTPA imaging only provides a ratio of 2.13 and without significant difference (p = 0.108). The results indicate that albumin-(Gd-DTPA) MBs have potential as a US/MR dual-modality contrast agent for BBB opening and differentiating focused-US-induced BBB opening from ICH, and can monitor the focused ultrasound brain drug delivery process. ? 2012 Institute of Physics and Engineering in Medicine. | - |
dc.relation.ispartof | Physics in Medicine Biology | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | Blood-brain barrier; Contrast agent; Contrast-enhanced; Drug delivery process; Dual-modality; Focused ultrasound; Gd-DTPA; Imaging experiments; In-vitro; In-vivo; Mean diameter; Microbubbles; MR images; MR imaging; Perfluorocarbons; Signal intensities; Sodium salt; Ultrasound imaging; Xylenol Orange; Drug delivery; Imaging systems; Magnetic resonance; Magnetic resonance imaging; Paramagnetism; Rats; Ultrasonic imaging; Ultrasonics; Gadolinium; albuminoid; contrast medium; diagnostic agent; fluorocarbon; gadolinium pentetate; microsphere; animal; article; blood brain barrier; chemistry; drug delivery system; echography; feasibility study; human; magnetism; male; metabolism; methodology; nuclear magnetic resonance imaging; permeability; rat; Sprague Dawley rat; Albumins; Animals; Blood-Brain Barrier; Contrast Media; Drug Delivery Systems; Feasibility Studies; Fluorocarbons; Gadolinium DTPA; Humans; Magnetic Phenomena; Magnetic Resonance Imaging; Male; Microspheres; Permeability; Rats; Rats, Sprague-Dawley; Ultrasonography | - |
dc.title | Paramagnetic Perfluorocarbon-Filled Albumin-(Gd-DTPA) Microbubbles for the Induction of Focused-Ultrasound-Induced Blood─Brain Barrier Opening Concurrent MR Ultrasound Imaging | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1088/0031-9155/57/9/2787 | - |
dc.identifier.scopus | 2-s2.0-84860122228 | - |
dc.relation.pages | 2787-2802 | - |
dc.relation.journalvolume | 57 | - |
item.openairetype | journal article | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
crisitem.author.dept | Electrical Engineering | - |
crisitem.author.dept | Biomedical Electronics and Bioinformatics | - |
crisitem.author.dept | Electrical Engineering | - |
crisitem.author.orcid | 0000-0001-7603-7852 | - |
crisitem.author.parentorg | College of Electrical Engineering and Computer Science | - |
crisitem.author.parentorg | College of Electrical Engineering and Computer Science | - |
crisitem.author.parentorg | College of Electrical Engineering and Computer Science | - |
顯示於: | 電機工程學系 |
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