https://scholars.lib.ntu.edu.tw/handle/123456789/516811
標題: | MMP-3 response to compressive forces in vitro and in vivo | 作者: | HAO-HUENG CHANG Wu C.-B. ALEX YUNN-JY CHEN Weng C.-Y. Wong W.-P. Chang B.-E. BEI-EN CHANG MIN-HUEY CHEN JANE CHUNG-CHEN YAO YI-JANE CHEN |
公開日期: | 2008 | 卷: | 87 | 期: | 7 | 起(迄)頁: | 692-696 | 來源出版物: | Journal of Dental Research | 摘要: | During orthodontic tooth movement, bone resorption occurs at the compression site. However, the mechanism underlying resorption remains unclear. Applying compressive force to human osteoblast-like cells grown in a 3D collagen gel, we examined gene induction by using microarray and RT-PCR analysis. Among 43 genes exhibiting significant changes, cyclooxygenase-2, ornithine decarboxylase, and matrix metalloproteinase-3 (MMP-3) were up-regulated, whereas membrane-bound interleukin-1 receptor accessory protein was down-regulated. The MMP-3 protein increases were further confirmed by Western blot. To ascertain whether MMP-3 is up-regulated in vivo by orthodontic force, we examined human bone samples at the compressive site by realigning the angulated molars. Immunohistochemical staining revealed MMP-3 distributed along the compressive site of the bony region within 3 days of compression. Since MMP-3 participates in degradation of a wide range of extracellular matrix molecules, we propose that MMP-3 plays an important role in bone resorption during orthodontic tooth movement. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-47649108546&doi=10.1177%2f154405910808700714&partnerID=40&md5=0c4c3c281c1fe632df630acc45b1c324 https://scholars.lib.ntu.edu.tw/handle/123456789/516811 |
ISSN: | 0022-0345 | DOI: | 10.1177/154405910808700714 | SDG/關鍵字: | collagen; cyclooxygenase 2; ornithine decarboxylase; PTGS2 protein, human; stromelysin; adaptation; article; biological model; bone remodeling; cell culture; culture technique; cytology; DNA microarray; enzymology; gel; gene expression profiling; gene expression regulation; human; jaw; metabolism; nonparametric test; osteoblast; periodontal disease; physiology; Adaptation, Physiological; Alveolar Process; Bone Remodeling; Cell Culture Techniques; Cells, Cultured; Collagen; Cyclooxygenase 2; Gels; Gene Expression Profiling; Gene Expression Regulation; Humans; Matrix Metalloproteinase 3; Models, Biological; Oligonucleotide Array Sequence Analysis; Ornithine Decarboxylase; Osteoblasts; Statistics, Nonparametric; Tooth Movement |
顯示於: | 牙醫學系 |
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