An acute decrease in plasma membrane tension induces macropinocytosis via PLD2 activation
Journal
Journal of cell science
Journal Volume
132
Journal Issue
17
Date Issued
2019
Author(s)
Loh, Julie
Chuang, Mei-Chun
Lin, Shan-Shan
Joseph, Jophin
Su, You-An
Hsieh, Tsung-Lin
Chang, Yu-Chen
Liu, Allen P
Abstract
Internalization of macromolecules and membrane into cells through endocytosis is critical for cellular growth, signaling and plasma membrane (PM) tension homeostasis. Although endocytosis is responsive to both biochemical and physical stimuli, how physical cues modulate endocytic pathways is less understood. Contrary to the accumulating discoveries on the effects of increased PM tension on endocytosis, less is known about how a decrease of PM tension impacts on membrane trafficking. Here, we reveal that an acute decrease of PM tension results in phosphatidic acid (PA) production, F-actin and phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2]-enriched dorsal membrane ruffling and subsequent macropinocytosis in myoblasts. The PA production induced by decreased PM tension depends on phospholipase D2 (PLD2) activation via PLD2 nanodomain disintegration. Furthermore, the 'decreased PM tension-PLD2-macropinocytosis' pathway is prominent in myotubes, reflecting a potential mechanism of PM tension homeostasis upon intensive muscle stretching and relaxation. Together, we identify a new mechanotransduction pathway that converts an acute decrease in PM tension into PA production and then initiates macropinocytosis via actin and PI(4,5)P2-mediated processes.
Subjects
Endocytosis; Mechanical transduction; PLD2 nanodomain; Phosphatidic acid
Publisher
COMPANY BIOLOGISTS LTD
Type
journal article