https://scholars.lib.ntu.edu.tw/handle/123456789/517767
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Shen E.P. | en_US |
dc.contributor.author | HSIAO-SANG CHU | en_US |
dc.contributor.author | YI-TING HSIEH | en_US |
dc.contributor.author | WEI-LI CHEN | en_US |
dc.contributor.author | SHAN-CHWEN CHANG | en_US |
dc.contributor.author | FUNG-RONG HU | en_US |
dc.creator | Shen E.P.;Chu H.-S.;Hsieh Y.-T.;Wei-Li Chen;Chang S.-C.;Hu F.-R. | - |
dc.date.accessioned | 2020-10-22T07:41:40Z | - |
dc.date.available | 2020-10-22T07:41:40Z | - |
dc.date.issued | 2020 | - |
dc.identifier.issn | 1367-0484 | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85075388022&doi=10.1016%2fj.clae.2019.10.137&partnerID=40&md5=797f454d3c913a1ec5a923f28d81fe14 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/517767 | - |
dc.description.abstract | Purpose: To compare the sensitivity of two genotypes of P. aeruginosa to various disinfectant solutions and analyze the attached bacteria on worn cosmetic contact lenses (cosCLs). Methods: In this prospective study, healthy volunteers wore etafilcon (brown), nelfilcon (gray), or hilafilcon (black) cosCLs and microbial adhesion analysis was performed. A rub-off test determined pigment dislodgement. Disinfectant sensitivity to Optifree Replenish (Alcon), Optifree Pure Moist (Alcon), Renu Fresh (Bausch & Lomb), and AoSept Plus (Ciba Vision) was tested at various disinfection times and compared between various genotypes and Type III secretion (T3S) system mutants. Results: Of the 1152 cosCLs collected, 364 were culture positive (32%). The highest rate of culture-positive lens was hilafilcon (chi square, P = 0.0001). Hilafilcon also had a significantly greater number of isolates than etafilcon (P < 0.0001). Hilafilcon was the only lens to fail the rub-off test. Cytotoxic strains were significant more resistant to Renu Fresh than were invasive strains, even at 100% of recommended disinfection time (P = 0.0005). Of the tested disinfectants, Renu Fresh was significantly less effective in killing both genotypes of P. aeruginosa compared to AoSept Plus at all time points (25%, 50%, 75%, and 100% recommended disinfection time, P = 0.0001, 0.0001, 0.0005, and 0.0005, respectively). When the T3S system was dysfunctional, mutant strains were all susceptible to disinfectants (P = 0.0001 for both invasive and cytotoxic strains). Conclusion: Pseudomonas species is commonly found on cosCLs of asymptomatic individuals. Wearers of cosCLs that dislodge pigments may be predisposed to microbial contamination. Cytotoxic strains are more resistant to disinfectant solutions, especially to Renu Fresh. P. aeruginosa disinfectant resistance requires a functional T3S system. ? 2019 British Contact Lens Association | - |
dc.relation.ispartof | Contact Lens and Anterior Eye | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | contact lens solution; disinfectant agent; contact lens solution; adult; antibiotic sensitivity; Article; bactericidal activity; bacterium adherence; bacterium culture; bacterium identification; controlled study; cytotoxicity; disinfection; female; genotype; human; human experiment; microbial contamination; mutant; nonhuman; normal human; priority journal; prospective study; Pseudomonas aeruginosa; type III secretion system; bacterial count; contact lens; hydrophilic contact lens; Pseudomonas aeruginosa; Colony Count, Microbial; Contact Lens Solutions; Contact Lenses; Contact Lenses, Hydrophilic; Disinfectants; Humans; Prospective Studies; Pseudomonas aeruginosa | - |
dc.title | Analysis of P. aeruginosa disinfectant sensitivity and microbial adhesions to worn cosmetic contact lenses | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1016/j.clae.2019.10.137 | - |
dc.identifier.pmid | 31711847 | - |
dc.identifier.scopus | 2-s2.0-85075388022 | - |
dc.relation.pages | 338-344 | - |
dc.relation.journalvolume | 43 | - |
dc.relation.journalissue | 4 | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
crisitem.author.dept | Ophthalmology | - |
crisitem.author.dept | Division of Cornea | - |
crisitem.author.dept | Ophthalmology-NTUH | - |
crisitem.author.dept | Ophthalmology-NTUH | - |
crisitem.author.dept | Division of Retina | - |
crisitem.author.dept | Ophthalmology | - |
crisitem.author.dept | Ophthalmology | - |
crisitem.author.dept | Ophthalmology-NTUH | - |
crisitem.author.dept | Division of Cornea | - |
crisitem.author.dept | Internal Medicine | - |
crisitem.author.dept | Internal Medicine-NTUH | - |
crisitem.author.dept | Office of the Vice President | - |
crisitem.author.dept | Clinical Pharmacy | - |
crisitem.author.dept | Medical Education and Bioethics | - |
crisitem.author.dept | Ophthalmology | - |
crisitem.author.dept | Ophthalmology-NTUH | - |
crisitem.author.dept | Division of Cornea | - |
crisitem.author.orcid | 0000-0002-3391-7347 | - |
crisitem.author.orcid | 0000-0003-2258-154X | - |
crisitem.author.orcid | 0000-0002-1538-2414 | - |
crisitem.author.orcid | 0000-0001-6505-4139 | - |
crisitem.author.orcid | 0000-0002-2102-5317 | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | Ophthalmology-NTUH | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | Ophthalmology-NTUH | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | Ophthalmology-NTUH | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | Administrative Unit | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | Ophthalmology-NTUH | - |
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