https://scholars.lib.ntu.edu.tw/handle/123456789/518330
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | PEI-JEN LOU | en_US |
dc.contributor.author | WEN-PIN CHEN | en_US |
dc.contributor.author | CHIN-TARNG LIN | en_US |
dc.contributor.author | Chen H.-C. | en_US |
dc.contributor.author | Wu J.-C. | en_US |
dc.creator | Pei-Jen Lou;Chen W.-P;Lin C.-T;Chen H.-C;Wu J.-C. | - |
dc.date.accessioned | 2020-10-27T12:04:49Z | - |
dc.date.available | 2020-10-27T12:04:49Z | - |
dc.date.issued | 2000 | - |
dc.identifier.issn | 0730-2312 | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033788821&doi=10.1002%2f1097-4644%2820001215%2979%3a4%3c542%3a%3aAID-JCB30%3e3.0.CO%3b2-Q&partnerID=40&md5=77db5fc38774ba1a91bfd88d68475c9d | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/518330 | - |
dc.description.abstract | Taxol affects microtubule dynamics by promoting microtubule assembly. To obtain a better insight into possible cross-talk between the microtubule- and actin-based cytoskeletons, we studied the short-term effects of Taxol treatment on the expression of actin and the E-cadherin/catenin complex in the nasopharyngeal carcinoma cell line TW-039 using immunofluorescence, immunoprecipitation, and immunoblotting methods. Morphologic changes in actin filaments, including ventral actin dumps and perijunctional actin blebs, were seen at Taxol concentrations ?1 μM. Levels of detergent-soluble E-cadherin fell to 53% or 58% compared to controls in cells treated, respectively, with 1 or 5 μM Taxol, while levels of detergent-soluble β-catenin fell to 76% or 74%. Levels of the detergent-soluble pool of α- and γ-catenin and the detergent-insoluble pool of the E-cadherin/catenin complex were unchanged by Taxol treatment and no significant difference was seen in the levels of adenomatous polyposis coli or glycogen synthase-3β or tyrosine phosphorylation patterns. These results suggest that modulation of microtubule dynamics by Taxol may have effects on the expression of actin and the cytosolic E-cadherin and β-catenin in nasopharyngeal carcinoma cells through pathways not involving the phosphorylation of β-catenin. (C) 2000 Wiley-Liss, Inc. | - |
dc.relation.ispartof | Journal of Cellular Biochemistry | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | actin; beta catenin; paclitaxel; uvomorulin; actin filament; article; cancer cell culture; controlled study; cytosol; drug effect; microtubule assembly; molecular interaction; nasopharynx carcinoma; priority journal; protein phosphorylation; Actins; Adenomatous Polyposis Coli Protein; Antineoplastic Agents, Phytogenic; Ca(2+)-Calmodulin Dependent Protein Kinase; Cadherins; Cell Size; Cytoskeletal Proteins; Cytoskeleton; Cytosol; Human; Microtubules; Nasopharyngeal Neoplasms; Neoplasm Proteins; Paclitaxel; Phosphorylation; Support, Non-U.S. Gov't; Tumor Cells, Cultured; Tyrosine | - |
dc.title | Taxol reduces cytosolic E-cadherin and β-catenin levels in nasopharyngeal carcinoma cell line TW-039: Cross-talk between the microtubule- and actin-based cytoskeletons | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1002/1097-4644(20001215)79:4<542 | - |
dc.identifier.pmid | 10996845 | - |
dc.identifier.scopus | 2-s2.0-0033788821 | - |
dc.relation.pages | 542-556 | - |
dc.relation.journalvolume | 79 | - |
dc.relation.journalissue | 4 | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
crisitem.author.dept | Otolaryngology | - |
crisitem.author.dept | Otolaryngology-NTUH | - |
crisitem.author.dept | Pharmacology | - |
crisitem.author.dept | Pathology | - |
crisitem.author.orcid | 0000-0002-3383-8593 | - |
crisitem.author.orcid | 0000-0002-0143-5642 | - |
crisitem.author.orcid | 0000-0003-3928-1412 | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
顯示於: | 醫學系 |
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