Generation of 2 induced pluripotent stem cell lines derived from patients with Parkinson's disease carrying LRRK2 G2385R variant
Journal
Stem Cell Research
Journal Volume
28
Pages
1-5
Date Issued
2018
Author(s)
Abstract
Leucine rich repeat kinase (LRRK2) is the most prevalent genetic cause for Parkinson's disease. LRRK2 p.G2385R is an Asian specific genetic risk factor for sporadic Parkinson's disease. We generated two induced pluripotent stem cells (iPSCs), IBMS-iPSC-018-09 and IBMS-iPSC-020-01, from the peripheral blood mononuclear cells of two patients carrying LRRK2 p.G2385R variant by using the Sendai-virus delivery system. These iPSCs had a normal karyotype and exhibited pluripotency, such as an embryonic stem cell-like morphology, expression of pluripotent markers, and capacity to differentiate into three germ layers. This cellular model will provide a platform for pathophysiological studies of neurodegeneration in Parkinson's disease. ? 2018
SDGs
Other Subjects
leucine rich repeat kinase 2; marker; leucine rich repeat kinase 2; LRRK2 protein, human; adult; Article; Asian; cell structure; clinical article; embryonic stem cell; genetic risk; genetic variability; genetic variation; germ layer; human; human cell; induced pluripotent stem cell; karyotype; male; middle aged; Parkinson disease; peripheral blood mononuclear cell; priority journal; Sendai virus; cell culture technique; cell line; cytology; genetics; induced pluripotent stem cell; mutation; nucleotide sequence; Parkinson disease; pathology; procedures; reproducibility; Adult; Base Sequence; Cell Culture Techniques; Cell Line; Humans; Induced Pluripotent Stem Cells; Leucine-Rich Repeat Serine-Threonine Protein Kinase-2; Male; Middle Aged; Mutation; Parkinson Disease; Reproducibility of Results
Publisher
Elsevier B.V.
Type
journal article