https://scholars.lib.ntu.edu.tw/handle/123456789/520278
Title: | Evaluation of nasal and nasopharyngeal swab collection for the detection of Epstein-Barr virus in nasopharyngeal carcinoma | Authors: | Coghill A.E CHENG-PING WANG Verkuijilen S.A.W.M Yu K.J Hsu W.-L Middeldorp J.M Hildesheim A. |
Keywords: | biostatistics and bioinformatics; epidemiology; Epstein-Barr virus; statistical inference; virus classification | Issue Date: | 2018 | Publisher: | John Wiley and Sons Inc. | Journal Volume: | 90 | Journal Issue: | 1 | Start page/Pages: | 191-195 | Source: | Journal of Medical Virology | Abstract: | Epstein-Barr virus detection using nasopharyngeal swabs has been suggested as a potential screening test that could improve the specificity of current EBV-based serological assays. However, application requires insertion of the swab deep into the nasopharynx, a procedure not amenable to non-clinic screening. We reasoned that swabbing the more easily accessible nasal cavity might provide an appealing alternative for NPC detection. Patients > 18 years of age diagnosed with histologically confirmed NPC were recruited from the Otolaryngology Department at the National Taiwan University Hospital. ENT clinicians collected both nasal and nasopharyngeal swabs. EBV DNA and cellular beta-globulin DNA were quantified using quantitative PCR targeting a highly-conserved region of the BKRF1 gene. EBV DNA was detectable (non-zero) in all 34 nasopharyngeal swabs and above the positivity threshold of 1666 EBV copies in 30 (88.2%) patients. EBV DNA was detectable in 50% of 34 nasal swabs and above the positivity threshold in four (11.8%) patients. Average EBV DNA levels were >3-fold higher (P < 0.001) in nasopharyngeal compared to nasal swabs. Among the 17 NPC patients with detectable EBV DNA in both swab types, we observed correlation (P < 0.01) between EBV DNA measurements. Our data represent the first evaluation of EBV DNA collected from nasal swabs. Given current EBV DNA amplification techniques, nasopharyngeal swabs remain more sensitive than nasal swabs for NPC detection. ? 2017 Wiley Periodicals, Inc. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85029300286&doi=10.1002%2fjmv.24918&partnerID=40&md5=fe15300ee6c9b07a99bc10b141253999 https://scholars.lib.ntu.edu.tw/handle/123456789/520278 |
ISSN: | 0146-6615 | DOI: | 10.1002/jmv.24918 | SDG/Keyword: | beta globulin; DNA; Epstein Barr virus antigen 1; lidocaine; virus antigen; virus DNA; adult; age; Article; BKRF1 gene; cancer localization; cancer staging; clinical evaluation; controlled study; diagnostic test accuracy study; Epstein Barr virus; female; gender; gene; histology; human; major clinical study; male; middle aged; nasopharynx; nasopharynx carcinoma; nose cavity; nose smear; polymerase chain reaction; quantitative analysis; virus classification; virus detection; aged; carcinoma; classification; Epstein Barr virus; Epstein Barr virus infection; evaluation study; genetics; immunology; isolation and purification; nasopharynx; nasopharynx tumor; nose; procedures; real time polymerase chain reaction; sensitivity and specificity; specimen handling; Taiwan; virology; virus load; young adult; Adult; Aged; Antigens, Viral; Carcinoma; DNA, Viral; Epstein-Barr Virus Infections; Female; Herpesvirus 4, Human; Humans; Male; Middle Aged; Nasopharyngeal Neoplasms; Nasopharynx; Nose; Real-Time Polymerase Chain Reaction; Sensitivity and Specificity; Specimen Handling; Taiwan; Viral Load; Young Adult |
Appears in Collections: | 醫學院附設醫院 (臺大醫院) |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.