https://scholars.lib.ntu.edu.tw/handle/123456789/523452
Title: | A CTLA-4 Antagonizing DNA Aptamer with Antitumor Effect | Authors: | Huang B.-T. Lai W.-Y. Chang Y.-C. Wang J.-W. Yeh S.-D. Lin E.P.-Y. PAN-CHYR YANG |
Keywords: | aptamer; cancer; CTLA-4; immunotherapy | Issue Date: | 2017 | Publisher: | Elsevier Inc | Journal Volume: | 8 | Start page/Pages: | 520-528 | Source: | Molecular Therapy - Nucleic Acids | Abstract: | The successful translation of cytotoxic T lymphocyte antigen-4 (CTLA-4) blockade has revolutionized the concept of cancer immunotherapy. Although monoclonal antibody therapeutics remain the mainstream in clinical practice, aptamers are synthetic oligonucleotides that encompass antibody-mimicking functions. Here, we report a novel high-affinity CTLA-4-antagonizing DNA aptamer (dissociation constant, 11.84 nM), aptCTLA-4, which was identified by cell-based SELEX and high-throughput sequencing. aptCTLA-4 is relatively stable in serum, promotes lymphocyte proliferation, and inhibits tumor growth in cell and animal models. Our study demonstrates the developmental pipeline of a functional CTLA-4-targeting aptamer and suggests a translational potential for aptCTLA-4. ? 2017 The Author(s) |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85029349672&doi=10.1016%2fj.omtn.2017.08.006&partnerID=40&md5=14210cc6fa0038ca7d0f2a44ced59d53 https://scholars.lib.ntu.edu.tw/handle/123456789/523452 |
ISSN: | 2162-2531 | DOI: | 10.1016/j.omtn.2017.08.006 | SDG/Keyword: | antineoplastic agent; aptamer; CTLA 4 antagonizing DNA aptamer; cytotoxic T lymphocyte antigen 4; DNA; unclassified drug; animal cell; animal experiment; animal model; animal tissue; antineoplastic activity; Article; binding affinity; cancer inhibition; controlled study; cytotoxic T lymphocyte; dissociation constant; drug dose escalation; drug protein binding; high throughput sequencing; human; in vitro study; in vivo study; Lewis carcinoma; lymphocyte proliferation; mouse; nonhuman; priority journal; systematic evolution of ligands by exponential enrichment aptamer technique; tumor associated leukocyte; tumor microenvironment |
Appears in Collections: | 醫學系 |
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