https://scholars.lib.ntu.edu.tw/handle/123456789/523726
標題: | Anticancer effects of tanshinone I in human non-small cell lung cancer | 作者: | Lee C.-Y. Sher H.-F. HUEI-WEN CHEN Liu C.-C. Chen C.-H. Lin C.-S. PAN-CHYR YANG Tsay H.-S. Chen J.J.W. |
公開日期: | 2008 | 卷: | 7 | 期: | 11 | 起(迄)頁: | 3527-3538 | 來源出版物: | Molecular Cancer Therapeutics | 摘要: | Tanshinones are the major bioactive compounds of Salvia miltiorrhiza Bunge (Danshen) roots, which are used in many therapeutic remedies in Chinese traditional medicine. We investigated the anticancer effects of tanshinones on the highly invasive human lung adenocarcinoma cell line, CL1-5. Tanshinone I significantly inhibited migration, invasion, and gelatinase activity in macrophage-conditioned medium-stimulated CL1-5 cells in vitro and also reduced the tumorigenesis and metastasis in CL1-5-bearing severe combined immunodeficient mice. Unlike tanshinone IIA, which induces cell apoptosis, tanshinone I did not have direct cytotoxicity. Real-time quantitative PCR, luciferase reporter assay, and electrophoretic mobility shift assay revealed that tanshinone I reduces the transcriptional activity of interleukin-8, the angiogenic factor involved in cancer metastasis, by attenuating the DNA-binding activity of activator protein-1 and nuclear factor-κB in conditioned medium-stimulated CL1-5 cells. Microarray and pathway analysis of tumor-related genes identified the differentially expressed genes responding to tanshinone I, which may be associated with the Ras-mitogen-activated protein kinase and Rac1 signaling pathways. These results suggest that tanshinone I exhibits anticancer effects both in vitro and in vivo and that these effects are mediated at least partly through the interleukin-8, Ras-mitogen-activated protein kinase, and Rac1 signaling pathways. Although tanshinone I has a remarkable anticancer action, its potential anticoagulant effect should be noted and evaluated. Copyright ? 2008 American Association for Cancer Research. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-56249087235&doi=10.1158%2f1535-7163.MCT-07-2288&partnerID=40&md5=dc0cd36c81ec6ca69ae51b90840173de https://scholars.lib.ntu.edu.tw/handle/123456789/523726 |
ISSN: | 1535-7163 | DOI: | 10.1158/1535-7163.MCT-07-2288 | SDG/關鍵字: | cryptotanshinone; dexamethasone; gelatinase; immunoglobulin enhancer binding protein; interleukin 8; Rac1 protein; Ras protein; tanshinone; tanshinone 1; tanshinone 2a; transcription factor AP 1; unclassified drug; animal experiment; animal model; animal tissue; anticoagulation; antineoplastic activity; apoptosis; article; assay; cancer cell; carcinogenesis; cell invasion; cell migration; controlled study; cytotoxicity; DNA binding; enzyme activity; female; gel mobility shift assay; gene; human; human cell; in vitro study; in vivo study; lung adenocarcinoma; lung non small cell cancer; macrophage; metastasis; microarray analysis; mouse; nonhuman; nucleotide sequence; priority journal; real time polymerase chain reaction; SCID mouse; Animals; Antineoplastic Agents, Phytogenic; Apoptosis; Carcinoma, Non-Small-Cell Lung; Cell Line, Tumor; Cell Proliferation; Cell Survival; Humans; Interleukin-8; Lung Neoplasms; Mice; Mice, SCID; Models, Biological; Neoplasm Metastasis; Phenanthrenes; RNA, Messenger |
顯示於: | 醫學系 |
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