https://scholars.lib.ntu.edu.tw/handle/123456789/528672
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Chou C.-H. | en_US |
dc.contributor.author | YU-TSUNG HUANG | en_US |
dc.contributor.author | Hsu H.-L. | en_US |
dc.contributor.author | Lai C.-C. | en_US |
dc.contributor.author | Liao C.-H. | en_US |
dc.contributor.author | PO-REN HSUEH | en_US |
dc.date.accessioned | 2020-12-18T08:38:53Z | - |
dc.date.available | 2020-12-18T08:38:53Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 1027-3719 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/528672 | - |
dc.description.abstract | SETTlNG: A medical centre in Taipei, Taiwan. OBJECTIVE: To investigate the performance of an enzyme-linked immunosorbent assay (ELISA) using anti-early secreted antigenic target 6 and culture filtrate protein 10 antibodies (MeDiPro Mycobacterium tuberculosis Antigen ELISA) for the detection of M. tuberculosis in positive signals of Mycobacterium Growth Indicator Tubes (MGIT; BACTEC MGIT 960 system). DESIGN: A total of 208 consecutive clinical samples, including 185 respiratory specimens and 23 non-respiratory specimens, with positive signals in MGIT were analysed. The assay was performed on Days 1 and 7. The ELISA and conventional culture results were compared. RESULTS: Among the tubes with positive signals, 86 (41.3%) were M. tuberculosis, 55 (26.4%) were non-tuberculous mycobacteria and 67 (32.2%) were negative for mycobacteria. The sensitivity of the ELISA for tubes with positive signals (initial smear with positive acid-fast bacilli) on Days 1 and 7 was respectively 75.6% (70.5%) and 96.5% (97.7%), and the specificity was respectively 98.4% (100%) and 100% (100%). CONCLUSION: Our results show that the MeDiPro M. tuberculosis Antigen ELISA is a simple, rapid assay (<3 h) for M. tuberculosis antigen detection, especially on Day 7 of incubation with positive signals in the BACTEC MGIT 960 system. ? 2009 The Union. | en_US |
dc.relation.ispartof | International Journal of Tuberculosis and Lung Disease | en_US |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | bacterial antigen; protein antibody; acid fast bacterium; article; bacterium detection; bacterium identification; comparative study; controlled study; culture technique; diagnostic accuracy; diagnostic value; enzyme linked immunosorbent assay; health center; human; human tissue; major clinical study; Mycobacterium tuberculosis; priority journal; respiratory system; sample; smear; Taiwan; tube; Antigens, Bacterial; Bacterial Proteins; Enzyme-Linked Immunosorbent Assay; Humans; Mycobacterium tuberculosis; Peptide Fragments; Sensitivity and Specificity | - |
dc.title | Rapid identification of the Mycobacterium tuberculosis complex by an enzyme-linked immunosorbent assay | en_US |
dc.type | journal article | en |
dc.identifier.pmid | 19723380 | - |
dc.identifier.scopus | 2-s2.0-69149099101 | - |
dc.relation.pages | 996-1001 | en_US |
dc.relation.journalvolume | 13 | en_US |
dc.relation.journalissue | 8 | en_US |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
crisitem.author.dept | Laboratory Medicine-NTUH | - |
crisitem.author.dept | Laboratory Medicine | - |
crisitem.author.dept | Laboratory Medicine | - |
crisitem.author.dept | Laboratory Medicine-NTUH | - |
crisitem.author.orcid | 0000-0002-7805-4178 | - |
crisitem.author.orcid | 0000-0002-7502-9225 | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
顯示於: | 醫學院附設醫院 (臺大醫院) |
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