https://scholars.lib.ntu.edu.tw/handle/123456789/536941
DC 欄位 | 值 | 語言 |
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dc.contributor.author | Wu F.-Y. | en_US |
dc.contributor.author | Liao Y.-W. | en_US |
dc.contributor.author | JIA-FENG WU | en_US |
dc.contributor.author | HUEY-LING CHEN | en_US |
dc.contributor.author | HONG-YUAN HSU | en_US |
dc.contributor.author | MEI-HWEI CHANG | en_US |
dc.contributor.author | YEN-HSUAN NI | en_US |
dc.creator | Wu F.-Y.;Liao Y.-W.;Wu J.-F.;Chen H.-L.;Hsu H.-Y.;Mei-Hwei Chang;Ni Y.-H. | - |
dc.date.accessioned | 2021-01-04T07:32:21Z | - |
dc.date.available | 2021-01-04T07:32:21Z | - |
dc.date.issued | 2016 | - |
dc.identifier.issn | 1875-9572 | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84960837051&doi=10.1016%2fj.pedneo.2015.07.003&partnerID=40&md5=c2d4076b5cbe90095d376a12694dbecc | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/536941 | - |
dc.description.abstract | Background Hepatitis B surface antigen (HBsAg) and hepatitis B surface antibody (anti-HBs) were conventionally determined by enzyme immunoassays. We aimed to apply a rapid, simple, and accurate method to detect HBsAg and its antibody. Methods We collected 1463 serum samples from healthy volunteers, hepatitis B carriers, and children of HBsAg-positive mothers. The test card that we examined is a chromatographic immunoassay for the qualitative detection of either HBsAg or anti-HBs. We then compared the results of the test card to the results of the conventional enzyme-immunoassay method, which is regarded as a standard. Results In the use of the test card to check HBsAg, the sensitivity was 88.8% and the specificity was 100%. The median hepatitis B virus viral load was significantly higher in the true-positive group [103.71 copies/mL (range, 102-109.03 copies/mL)] than in the false-negative group [102 copies/mL (range, 102-103.26 copies/mL)] (p = 0.005). In those who were younger than 2 years, the diagnostic accuracy of the HBsAg test card was 100%. Then, 1272 samples were tested for anti-HBs rapid test card. The sensitivity was 91.8% and the specificity was 96.5%. The median anti-HBs titer was significantly higher in the true-positive group (295.8 mIU/mL) than in the false-negative group (42.3 mIU/mL; p < 0.001). Conclusion Because of (1) the limited amount of blood sample required and (2) most of the young hepatitis B virus carriers having high viremia, and no concerns of false negativity, the test card is a good rapid screening tool for the detection of HBsAg and anti-HBs in pediatric group. ? 2015, Taiwan Pediatric Association. Published by Elsevier Taiwan LLC. This is an open access article under the CC BY-NC-ND license. | - |
dc.publisher | Elsevier (Singapore) Pte Ltd | - |
dc.relation.ispartof | Pediatrics and Neonatology | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | hepatitis B surface antibody; hepatitis B surface antigen; virus DNA; hepatitis B antibody; hepatitis B surface antigen; antibody detection; antigen detection; Article; blood sampling; child; controlled study; diagnostic accuracy; diagnostic test accuracy study; enzyme immunoassay; female; hepatitis B; hepatitis B rapid test; Hepatitis B virus; human; immunoaffinity chromatography; infant; major clinical study; male; sensitivity and specificity; viremia; virus carrier; virus load; blood; comparative study; enzyme linked immunosorbent assay; immunoassay; Taiwan; Enzyme-Linked Immunosorbent Assay; Female; Hepatitis B Antibodies; Hepatitis B Surface Antigens; Humans; Immunoassay; Infant; Male; Sensitivity and Specificity; Taiwan | - |
dc.title | A Simple and Rapid Test-card Method to Detect Hepatitis B Surface Antigen and Antibody: Potential Application in Young Children and Infants | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1016/j.pedneo.2015.07.003 | - |
dc.identifier.pmid | 26560182 | - |
dc.identifier.scopus | 2-s2.0-84960837051 | - |
dc.relation.pages | 219-224 | - |
dc.relation.journalvolume | 57 | - |
dc.relation.journalissue | 3 | - |
item.openairetype | journal article | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
crisitem.author.dept | Pediatrics | - |
crisitem.author.dept | Pediatrics-NTUH | - |
crisitem.author.dept | Medical Education and Bioethics | - |
crisitem.author.dept | Pediatrics-NTUH | - |
crisitem.author.dept | Pediatrics | - |
crisitem.author.dept | Medical Education and Bioethics | - |
crisitem.author.dept | Pediatrics-NTUH | - |
crisitem.author.dept | Pediatrics-NTUH | - |
crisitem.author.dept | Pediatrics | - |
crisitem.author.dept | Pediatrics | - |
crisitem.author.dept | Pediatrics-NTUH | - |
crisitem.author.orcid | 0000-0001-6343-1658 | - |
crisitem.author.orcid | 0000-0002-4074-5838 | - |
crisitem.author.orcid | 0000-0002-5720-4835 | - |
crisitem.author.orcid | 0000-0002-3648-9261 | - |
crisitem.author.orcid | 0000-0002-1158-5249 | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
顯示於: | 醫學系 |
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