https://scholars.lib.ntu.edu.tw/handle/123456789/537235
標題: | Analysis of integrated hepatitis B virus DNA and flanking cellular sequences in a childhood hepatocellular carcinoma | 作者: | Tsuei D.J. Hsu T.Y. Chen J.Y. MEI-HWEI CHANG Hsu H.C. Yang C.S. |
關鍵字: | childhood hepatocellular carcinoma; hepatitis B virus; hepatocarcinogene?sis; repetitive squences; trans?activation | 公開日期: | 1994 | 卷: | 42 | 期: | 3 | 起(迄)頁: | 287-293 | 來源出版物: | Journal of Medical Virology | 摘要: | The DNA of tumor tissue K1 obtained at autopsy from a case of hepatocellular carcinoma (HCC) in a 9?year?old boy contained integrated hepatitis B virus (HBV) DNA at a single site in the chromosome (case 2, Chang et al.: Hepatology 13:316?320, 1991). To characterize further the integrated viral DNA sequences, a genomic library of the K1 DNA was constructed in the lambda L47.1 vector. One phage clone, designated KTM?1, containing integrated HBV DNA and cellular flanking sequences was obtained from this library. The restriction map and DNA sequence of this clone showed that the integrated HBV DNA was partially deleted and rearranged. The most conserved viral DNA sequences were surface and X genes and arranged in the opposite orientation. The viral core gene was not present. Using chloramphenicol acetyltransferase (CAT) assay, the C?terminal truncated X open reading frame was demonstrated to retain its trans?activating ability. The result suggested that the functional integrated X gene may play a role in hepatocarcinogenesis. The study also showed that the right cellular flanking sequences were human alphoid repetitive sequences. ? 1994 Wiley?Liss, Inc. Copyright ? 1994 Wiley?Liss, Inc., A Wiley Company |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0028348904&doi=10.1002%2fjmv.1890420316&partnerID=40&md5=75deef601d1ba76fb2984b8ef5903f6c https://scholars.lib.ntu.edu.tw/handle/123456789/537235 |
ISSN: | 0146-6615 | DOI: | 10.1002/jmv.1890420316 | SDG/關鍵字: | article; carcinogenesis; child; hepatitis b; hepatitis b virus; human; human tissue; liver cell carcinoma; male; restriction mapping; transactivation; Base Sequence; Carcinoma, Hepatocellular; Child; Comparative Study; DNA, Neoplasm; DNA, Viral; Gene Expression Regulation, Neoplastic; Gene Expression Regulation, Viral; Hepatitis B; Hepatitis B Virus; Human; Liver Neoplasms; Male; Molecular Sequence Data; Open Reading Frames; Sequence Homology, Nucleic Acid; Support, Non-U.S. Gov't; Trans-Activation (Genetics); Virus Integration |
顯示於: | 醫學系 |
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