https://scholars.lib.ntu.edu.tw/handle/123456789/539756
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Liu C.W. | en_US |
dc.contributor.author | WEN-JEN LIN | en_US |
dc.creator | Liu C.W.;Wen-Jen Lin | - |
dc.date.accessioned | 2021-01-07T07:17:21Z | - |
dc.date.available | 2021-01-07T07:17:21Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 11769114 | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84870330031&doi=10.2147%2fIJN.S32830&partnerID=40&md5=7e0a4152224a5c5bd7baed23b1bd59d0 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/539756 | - |
dc.description.abstract | Background: This study was performed to develop a functional poly(D,L-lactide-co-glycolide)-poly(ethylene glycol) (PLGA-PEG)-bearing amino-active end group for peptide conjugation. Methods and results: PLGA was preactivated following by copolymerization with PEG diamine. The resulting amphiphilic PLGA-PEG copolymer bearing 97.0% of amino end groups had a critical micelle concentration of 3.0 × 10-8 mol/L, and the half-effective inhibition concentration (IC50) of the prepared PLGA-PEG nanoparticles was >100 mg/mL, which was much higher than that of PLGA nanoparticles (1.02 ± 0.37 mg/mL). The amphiphilic properties of PLGA-PEG spontaneously formed a core-shell conformation in the aqueous environment, and this special feature provided the amino group on the PEG chain scattered on the surface of PLGA-PEG nanoparticles for efficient peptide conjugation. The peptide-conjugated PLGA-PEG nanoparticles showed three-fold higher uptake than peptide-free PLGA-PEG nanoparticles in a SKOV3 cell line with high expression of epidermal growth factor receptor. Both peptide-conjugated and peptide-free PLGA-PEG nanoparticles were used as nanocarriers for delivery of doxorubicin. Although the rate of release of doxorubicin from both nanoparticles was similar, drug release at pH 4.0 (500 U lipase) was faster than at pH 7.4. The IC50 of doxorubicin-loaded peptide-conjugated PLGA-PEG nanoparticles in SKOV3 cells (0.05 ± 0.03 μg/mL) was much lower (by 62.4-fold) than that of peptide-free PLGA-PEG nanoparticles (3.12 ± 1.44 μg/mL). Conclusion: This in vivo biodistribution study in SKOV3 tumor-bearing mice was further promising in that accumulation of doxorubicin in tumor tissue was in the order of peptide-conjugated PLGA-PEG nanoparticles > peptide-free PLGA-PEG nanoparticles > doxorubicin solution. ? 2012 Liu and Lin publisher and licensee Dove Medical Press Ltd. | - |
dc.relation.ispartof | International Journal of Nanomedicine | - |
dc.subject | Amphiphilic copolymer; Doxorubicin; Nanoparticles; Peptide; SKOV3 cell | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | doxorubicin; epidermal growth factor receptor; heptapeptide; macrogol; nanoparticle; polyglactin; animal experiment; animal model; animal tissue; antiproliferative activity; article; cancer inhibition; cell viability; controlled study; drug accumulation; drug delivery system; drug distribution; drug dosage form comparison; drug effect; drug formulation; drug mechanism; drug release; drug targeting; female; IC 50; in vivo study; micelle; mouse; nonhuman; pH measurement; polymerization; protein expression; surface property; Animals; Antibiotics, Antineoplastic; Cell Line, Tumor; Doxorubicin; Epidermal Growth Factor; Female; Ligands; Metabolic Clearance Rate; Mice; Mice, Nude; Nanocapsules; Organ Specificity; Ovarian Neoplasms; Peptides; Polyethylene Glycols; Polyglactin 910; Tissue Distribution; Mus | - |
dc.title | Polymeric nanoparticles conjugate a novel heptapeptide as an epidermal growth factor receptor-active targeting ligand for doxorubicin | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.2147/IJN.S32830 | - |
dc.identifier.pmid | 22973097 | - |
dc.identifier.scopus | 2-s2.0-84870330031 | - |
dc.relation.pages | 4749-4767 | - |
dc.relation.journalvolume | 7 | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.cerifentitytype | Publications | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
crisitem.author.dept | Pharmacy | - |
crisitem.author.dept | School of Pharmacy | - |
crisitem.author.orcid | 0000-0001-9879-1071 | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
顯示於: | 藥學系 |
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