https://scholars.lib.ntu.edu.tw/handle/123456789/544373
標題: | XRCC1 Arg194Trp and Arg399Gln polymorphisms and arsenic methylation capacity are associated with urothelial carcinoma | 作者: | Chiang C.-I. Huang Y.-L. Chen W.-J. Shiue H.-S. CHAO-YUAN HUANG YEONG-SHIAU PU Lin Y.-C. Hsueh Y.-M. |
公開日期: | 2014 | 出版社: | Academic Press Inc. | 卷: | 279 | 期: | 3 | 起(迄)頁: | 373-379 | 來源出版物: | Toxicology and Applied Pharmacology | 摘要: | The association between DNA repair gene polymorphisms and bladder cancer has been widely studied. However, few studies have examined the correlation between urothelial carcinoma (UC) and arsenic or its metabolites. The aim of this study was to examine the association between polymorphisms of the DNA repair genes, XRCC1 Arg194Trp, XRCC1 Arg399Gln, XRCC3 Thr241Met, and XPD Lys751Gln, with urinary arsenic profiles and UC. To this end, we conducted a hospital-based case-control study with 324 UC patients and 647 age- and gender-matched non-cancer controls. Genomic DNA was used to examine the genotype of XRCC1 Arg194Trp, XRCC1 Arg399Gln, XRCC3 Thr241Met, and XPD Lys751Gln by PCR-restriction fragment length polymorphism analysis (PCR-RFLP). Urinary arsenic profiles were measured by high performance liquid chromatography (HPLC) linked with hydride generator and atomic absorption spectrometry. The XRCC1 399 Gln/. Gln and 194 Arg/. Trp and Trp/. Trp genotypes were significantly related to UC, and the odds ratio (OR) and 95% confidence interval (95%CI) were 1.68 (1.03-2.75) and 0.66 (0.48-0.90), respectively. Participants with higher total urinary arsenic levels, a higher percentage of inorganic arsenic (InAs%) and a lower percentage of dimethylarsinic acid (DMA%) had a higher OR of UC. Participants carrying XRCC1 risk diplotypes G-C/G-C, A-C/A-C, and A-T/G-T, and who had higher total arsenic levels, higher InAs%, or lower DMA% compared to those with other XRCC1 diplotypes had a higher OR of UC. Our results suggest that the XRCC1 399 Gln/. Gln and 194 Arg/. Arg DNA repair genes play an important role in poor arsenic methylation capacity, thereby increasing the risk of UC in non-obvious arsenic exposure areas. ? 2014 Elsevier Inc. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84907195402&doi=10.1016%2fj.taap.2014.06.027&partnerID=40&md5=f1c456b3d37375233bf7826ae250e4be https://scholars.lib.ntu.edu.tw/handle/123456789/544373 |
ISSN: | 0041-008X | DOI: | 10.1016/j.taap.2014.06.027 | SDG/關鍵字: | arginine; arsenic; cacodylic acid; genomic DNA; glutamine; lysine; methionine; threonine; tryptophan; xeroderma pigmentosum group D protein; XRCC1 protein; XRCC3 protein; adult; Article; atomic absorption spectrometry; bladder carcinogenesis; chemical carcinogenesis; controlled study; female; genetic association; genotype; heterozygote; high performance liquid chromatography; hospital based case control study; human; major clinical study; male; methylation; polymerase chain reaction; restriction fragment length polymorphism; single nucleotide polymorphism; transitional cell carcinoma; urinalysis; urine level; Arsenic; Arsenic methylation capacity; Polymorphism; Urothelial carcinoma; XRCC1; Aged; Arsenic; Cacodylic Acid; Case-Control Studies; Chromatography, High Pressure Liquid; Confidence Intervals; DNA; DNA Repair; DNA-Binding Proteins; Environmental Exposure; Female; Genotype; Humans; Male; Methylation; Middle Aged; Odds Ratio; Poisons; Polymorphism, Genetic; Questionnaires; Real-Time Polymerase Chain Reaction; Risk Factors; Socioeconomic Factors; Spectrophotometry, Atomic; Urologic Neoplasms; Xeroderma Pigmentosum Group D Protein |
顯示於: | 醫學系 |
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