https://scholars.lib.ntu.edu.tw/handle/123456789/546833
標題: | Effects of Human Umbilical Cord-Derived Mesenchymal Stem Cells on the Acute Cigarette Smoke-Induced Pulmonary Inflammation Model | 作者: | Chen, X.-Y. Chen, Y.-Y. Lin, W. Chien, C.-W. Chen, C.-H. Wen, Y.-C. Hsiao, T.-C. Chuang, H.-C. TA-CHIH HSIAO |
關鍵字: | cigarette smoke; inflammation; lung; mesenchymal stem cells; oxidative stress | 公開日期: | 2020 | 卷: | 11 | 來源出版物: | Frontiers in Physiology | 摘要: | Cigarette smoke (CS) has been reported to induce oxidative stress and inflammatory process in the lungs. However, the role of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) in the regulation of pulmonary inflammation remains unclear. The objective of this study is to investigate the effects of hUC-MSCs on lung inflammation in the acute CS-induced pulmonary inflammation animal model. Eight-week-old male C57BL/6 mice were intravenously administered 3 × 106, 1 × 107, and 3 × 107 cells/kg of hUC-MSCs as well as normal saline alone (control) after 3 days of CS exposure. Mice exposed to high-efficiency particulate air (HEPA)-filtered room air served as the CS control group. High-dose (3 × 107 cells/kg) hUC-MSC administration significantly decreased tumor necrosis factor (TNF)-α in the bronchoalveolar lavage fluid (BALF) of CS-exposed mice (p < 0.05). The chemokine (CXC motif) ligand 1/keratinocyte chemoattractant (CXCL1/KC) in BALF were significantly reduced by low-dose (3 × 106 cells/kg) and high-dose (3 × 107 cells/kg) hUC-MSC (p < 0.05). Medium-dose hUC-MSC administration decreased interleukin (IL)-1β in lung of mice, and TNF-α and caspase-3 were decreased in the lung of CS-exposed mice by medium- and high-dose MSC (p < 0.05). Low-dose hUC-MSCs significantly elevated serum CXCL1/KC and IL-1β in CS-exposed mice (p < 0.05). Our results suggest that high-dose hUC-MSCs reduced pulmonary inflammation and had antiapoptotic effects in acute pulmonary inflammation. ? Copyright ? 2020 Chen, Chen, Lin, Chien, Chen, Wen, Hsiao and Chuang. |
URI: | https://www.scopus.com/inward/record.url?eid=2-s2.0-85089938544&partnerID=40&md5=d40970c992c178f6bd0a84ecba5d9d05 https://scholars.lib.ntu.edu.tw/handle/123456789/546833 |
DOI: | 10.3389/fphys.2020.00962 | SDG/關鍵字: | caspase 3; CXCL1 chemokine; interleukin 1beta; tumor necrosis factor; acute disease; animal experiment; animal model; animal tissue; antiapoptotic activity; Article; body weight; bronchoalveolar lavage fluid; C57BL 6 mouse; cigarette smoking; controlled study; enzyme linked immunosorbent assay; human; human cell; lung injury; lung parenchyma; male; mesenchymal stem cell transplantation; mouse; nonhuman; particulate matter; pneumonia; protein motif; therapy effect; umbilical cord |
顯示於: | 環境工程學研究所 |
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