https://scholars.lib.ntu.edu.tw/handle/123456789/548566
標題: | Olfactory ensheathing cells improve the survival of porcine neural xenografts in a Parkinsonian rat model | 作者: | Weng, S.-J. Chen, C.-F.F. Huang, Y.-S. Chiu, C.-H. Wu, S.-C. Lin, C.-Y. Chueh, S.-H. Cheng, C.-Y. Ma, K.-H. SHINN-CHIH WU |
關鍵字: | olfactory ensheathing cell; porcine ventral mesencephalon; rat model of Parkinson's disease; xenograft | 公開日期: | 2020 | 卷: | 27 | 期: | 2 | 來源出版物: | Xenotransplantation | 摘要: | Background: Parkinson's disease (PD) features the motor control deficits resulting from irreversible, progressive degeneration of dopaminergic (DA) neurons of the nigrostriatal pathway. Although intracerebral transplantation of human fetal ventral mesencephalon (hfVM) has been proven effective at reviving DA function in the PD patients, this treatment is?clinically limited by availability of hfVM?and the related?ethical issues. Homologous tissues?to hfVM, such as porcine fetal ventral mesencephalon (pfVM) thus present a strong clinical potential if immune response following xenotransplantation could be tamed. Olfactory ensheathing cells (OECs) are glial cells showing immunomodulatory properties. It is unclear but intriuging whether these properties can be applied to reducing immune response following neural xenotransplantation of PD. Methods: To determine whether OECs may benefit neural xenografts for PD, different compositions of grafting cells?were transplanted into striatum of the PD model rats. We used apomorphine-induced rotational behavior to evaluate effectiveness of the neural grafts on reviving DA function. Immunohistochemistry was applied to investigate the effect of OECs on the survival of neuroxenografts and underlying?mechanisms of?this effect. Results: Four weeks following the xenotransplantation, we found that the PD rats receiving pfVM?+?OECs co-graft exhibited a better improvement in apomorphine-induced rotational behavior compared with those receiving only pfVM cells. This result can be explained by higher survival of DA neurons (tyrosine hydroxylase immunoreactivity) in grafted striatum of pfVM?+?OECs group. Furthermore, pfVM?+?OECs group has less immune response (CD3+ T cells and OX-6+ microglia) around the grafted area compared with pfVM only group. These results suggest that OECs may enhance the survival of the striatal xenografts?via dampening?the?immune response?at the grafted sites. Conclusions: Using allogeneic OECs as a co-graft material for xenogeneic neural grafts could be a feasible therapeutic strategy to enhance results and applicability of the cell replacement therapy for PD. ? 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. |
URI: | https://www.scopus.com/inward/record.url?eid=2-s2.0-85075757460&partnerID=40&md5=f539dad035f715b1eff45a6146c8a185 https://scholars.lib.ntu.edu.tw/handle/123456789/548566 |
DOI: | 10.1111/xen.12569 | SDG/關鍵字: | tyrosine 3 monooxygenase; dopamine; animal cell; animal experiment; animal model; animal tissue; Article; CD3+ T lymphocyte; cell survival; cell transplantation; circling behavior; controlled study; corpus striatum; dopaminergic activity; dopaminergic nerve cell; female; fetus; graft survival; immune response; male; microglia; nerve graft; newborn; nonhuman; olfactory ensheathing cell transplantation; Parkinson disease; pig; priority journal; rat; therapy effect; ventral mesencephalon; xenotransplantation; animal; cell transplantation; cytology; disease model; fetal tissue transplantation; immunology; mesencephalon; metabolism; olfactory bulb; Parkinson disease; procedures; Sprague Dawley rat; transplantation; xenograft; Animals; Cell Transplantation; Disease Models, Animal; Dopamine; Fetal Tissue Transplantation; Heterografts; Male; Mesencephalon; Olfactory Bulb; Parkinson Disease; Rats, Sprague-Dawley; Transplantation, Heterologous |
顯示於: | 動物科學技術學系 |
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