https://scholars.lib.ntu.edu.tw/handle/123456789/550879
標題: | K-ras mutation at codon 12 in stage I pancreatic adenocarcinoma: Analysis by laser capture microdissection and direct sequencing | 作者: | MING-CHU CHANG YU-TING CHANG MING-SHIANG WU CHIA-TUNG SHUN YU-WEN TIEN Lin J.-T. |
公開日期: | 2001 | 卷: | 100 | 期: | 5 | 起(迄)頁: | 352-354 | 來源出版物: | Journal of the Formosan Medical Association | 摘要: | Pancreatic ductal adenocarcinoma has been reported to carry a rate mutation high in codon 12 of the K-ras oncogene. To avoid the pitfalls of conventional methods of tissue dissection that might affect the sensitivity and specificity of detecting K-ras mutation, laser capture microdissection (LCM) technique was used. Pancreatic adenocarcinoma tissues were obtained from 15 patients who underwent Whipple's procedure. Selected tissues procured by LCM were analyzed by direct sequencing after polymerase chain reaction amplification of K-ras sequences at codon 12. K-ras mutation was noted in nine patients. All mutations showed G to A substitution at codon 12. The mutational pattern (GGT to GAT) is similar in both western and eastern reports. LCM is a feasible method to effectively obtain pure tumor cells from a surgical specimen. It remains to be determined whether this low mutation rate is a result of relatively early stage of disease or different carcinogenesis in different geographic regions. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034966321&partnerID=40&md5=d82ab5fb58dd083cea6608eeafb242cf https://scholars.lib.ntu.edu.tw/handle/123456789/550879 |
ISSN: | 0929-6646 | SDG/關鍵字: | adenine; guanine; thymine; adult; aged; article; cancer cell; cancer staging; carcinogenesis; clinical article; codon; controlled study; Eastern Hemisphere; female; gene mutation; gene sequence; genetic analysis; geography; human; human tissue; laser capture microdissection; male; mutation rate; nucleic acid base substitution; oncogene K ras; pancreas adenocarcinoma; pancreas duct; pancreaticoduodenectomy; polymerase chain reaction; sensitivity and specificity; technique; tissue section; Western Hemisphere; Adenocarcinoma; Adult; Aged; Cell Separation; Codon; Female; Genes, ras; Humans; Lasers; Male; Middle Aged; Pancreatic Neoplasms; Point Mutation; Sequence Analysis; Tumor Markers, Biological |
顯示於: | 醫學系 |
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